This research investigated the application of organizational learning and cooperation network on the accumulation of resource bases for sustaining the competitive superiority of enterprise. A company which moved from the traditional Chinese herbal medicine industry to biotechnology industry was chosen for our case study. The results showed the company retained good network relationships to obtain required resources and core competence, including product knowledge and technologies, as well as government funds and marketing channels, even receiving an award. Moreover, the company built effective organizational learning through industry-government-university collaboration. It was therefore beneficial to technology innovation, product development, personnel training and business growth. The company also subcontracted some products for utilizing less investment resources to rapidly expand business scale. Thus, organizational learning and cooperation networks can enable enterprises to strengthen their competitiveness and successfully transform themselves into excellence performers.