Dynamic Evaluation of 18F-FDG Uptake by MicroPET and Whole-Body Autoradiography in a Fibrosarcoma-Bearing House Model

以微正子斷層造影及全身自體射線攝影動態評估氟－18去氧葡萄糖在纖維肉芽瘤小鼠動物模式之吸收

張志賢(Chih-Hsien Chang) ； 范國賢(Kuo-Hsien Fan) ； 陳德容(Te-Jung Chen) ； 徐維荃(Wei-Chun Hsu) ； 詹美齡(Mei-Ling Jan) ； 蔡東湖(Tung-Hu Tsai) ； 高潘福(Pan-Fu Kao) ； 陳介甫(Chieh-Fu Chen) ； 傅應凱(Ying-Kai Fu) ； 李德偉(Te-Wei Lee)

《Journal of the Formosan Medical Association》 103卷11期 (2004/11) Pp. 876-881

https://doi.org/10.29828/JFMA.200411.0012

並列摘要

Background and Purpose: Fluorine-18-2-deoxy-D-glucose (18F-FDG) has been used in the clinic as a diagnostic radiotracer for monitoring many kinds of tumors, but its value for monitoring fibrosarcoma is not well established. Methods: In this study, the uptake of 18F-FDG in a fibrosarcoma-bearing mouse model was evaluated using the high resolution positron emission tomography (PET) system microPET. Tumor cells were implanted in 3 FVB/N mice, and static microPET scanning was performed on day 1, 7, 12 and 15 after implantation. A dynamic microPET image was scanned on day 12 to determine the 18F-FDG uptake in 3 other tumor-bearing mice. Time-activity curves were plotted by drawing regions of interest in the tumor, liver, kidneys and muscles. The mice were sacrificed after dynamic microPET imaging and whole-body autoradiography (WBAR) was performed. For biodistribution study, 9 tumor-bearing mice, 3 per experimental group, were studied at 3 time points and the results were compared with the static microPET images. Results: MicroPET images suggested that 18F-FDG could be used to monitor the growth of tumors 7 days after implantation. Dynamic scans of 18F-FDG uptake reached a plateau in the tumor after 20 minutes on day 12 after implantation. Both microPET and WBAR revealed evidence of tumor necrosis. The results of biodistribution and WBAR agreed with those from microPET images.
Conclusion: MicroPET was useful for monitoring the growth of fibrosarcoma and determination of the maximal uptake time point of 18F-FDG in tumors in this tumor-bearing mouse model.

並列關鍵字

Autoradiography ； Fibrosarcoma ； Fludeoxyglucose F 18 ； Tomography ； emission computed