Areca Quid Chewing Enhances the Expression of Salivary Matrix Metalloproteinase-9

檳榔塊的咀嚼增加唾液中第九型基質金屬蛋白酶的表現

劉巡宇(Shyun-Yeu Liu) ； 林美惠(Mei-Huei Lin) ； 楊舜鈞(Shun-Chun Yang) ； 黃冠誠(Guan-Cheng Huang) ； 張王麗雪(Lisa Chang) ； 張善鈞(Sam Chang) ； 顔欽堉(Ching-Yu Yen) ； 蔣維凡(Wei-Fan Chiang) ； 李今海(Chin-Hai Lee) ； 郭永煙(Yung-Yen Kuo) ； 劉永超(Young-Chau)

《Journal of the Formosan Medical Association》 104卷2期 (2005/02) Pp. 113-119

https://doi.org/10.29828/JFMA.200502.0007

並列摘要

Background and Purpose: The effects of areca quid (AQ) consumption on salivary matrix metalloproteinases (MMPs) which may participate in tumor invasion and metastasis remains unclear. This study assessed the change in salivary MMP-9 protein levels 2 hours after 5-minute AQ chewing stimulation (AQCS) in non-AQ users and the expression profile of this proteinase in saliva and tumor specimens of oral squamous cell carcinoma (OSCC) patients with a history of AQ use.
Methods: MMP-9 transcript was analyzed by reverse transcription-polymerase chain reaction. MMP-9 protein level was measured by both Western blot and gelatin zymography.
Results: The protein level of salivary MMP-9 was 3.1-to 8.9-fold enhanced 2 h after AQCS in 3 healthy volunteers as revealed by Western blot and zymography. As a control, gum chewing did not significantly change salivary MMP-9 protein level. Expression of MMP-9 transcript was found in 25 of 28 OSCC specimens and significantly correlated with cervical lymph node metastasis (p=0.037). All of the 8 tested OSCC tissue homogenate samples available and all 12 saliva samples from 12 oral tumor outpatients were positive for MMP-9 protein.
Conclusions: Elevation of MMP-9 may be one of the net effects of AQCS in vivo, which may play a role in the pathogenesis of oral mucosal lesions. Furthermore, the association of MMP-9 expression with neck-lymph-node metastasis may imply a significant role of MMP-9 in the progression of OSCC among patients with a history of AQ use in Taiwan.

並列關鍵字

Arecoline ； Gelatinase B ； Matrix metalloproteinases ； Saliva