長柄菊萃取物之生物活性探討

Studies on Biological activity of extracts Tridax procumbens Linn.

楊家偉(Jia- Wei Yang)

指導教授 ： 韓建國 ； 趙哲毅 ； 黃晉修

亞洲大學/健康學院/保健營養生技學系碩士班/碩士(2011年)

摘要

長柄菊（Tridax procumbens Linn.），為菊科多年生草本植物，亦是坊間青草茶常用之原料，具有清熱解毒，抗菌及抗腫瘤作用。本研究以長柄菊的花 (Flower) 、莖 (Stem)、葉 (Leave) 為原料，分別測試其抗氧化力、抑菌作用、抗發炎能力與抗肝癌效用。
實驗結果發現長柄菊各萃取層都具有抗氧化能力(DPPH、還原力、亞鐵離子螯合、總酚)，在DPPH清除自由基清除能力中以花乙醇層能力最佳，清除效果達88 %，其總酚含量總酚含量可達 120 mg/g；而螯合亞鐵離子能力則是以水層較佳螯合能力達 55%。抑菌試驗結果發現，不同部位之乙酸乙酯層，其對於李斯特菌具顯著的抑制作用，其中又以花之乙酸乙酯層效果較佳，抑菌圈達1.23 ±0.23cm。抗發炎介質nitric oxide (NO)方面，以花之乙酸乙酯有較佳的抑制效果能抑制76% NO的產生。抗肝癌試驗發現，花、莖及葉對肝癌細胞(SK-Hep-1)會有影響肝癌生長能力。在細胞轉移試驗結果發現，在轉移、侵襲性實驗及抑制癌細胞分泌侵襲因子表現上，花的乙醇萃取物有較佳，能降低17 % MMP-9表現量及12 % MMP-2表現量。綜合上述，在抗癌作用上有較佳的效果，且證實了花乙醇萃取物有抗肝癌細胞之轉移作用及抑制其生長的效果。

關鍵字

長柄菊 ； 抑菌 ； 抗氧化 ； 抗發炎 ； 抗癌

並列摘要

Tridax procumbens Linn. is used in folk medicine. It is reported that the plant shows antibacterial activity and contains antitumor chemical components. The purpose of this research was to evaluate antibacterial, antioxidation, anti-inflammation and anticancer activity of T. procumbens Linn. The flowers , leaves and stems of T. procumbens were extracted with ten times of ethyl alcohol. The extract was then partitioned by n-hexane, ethyl acetate, n-butyl alcohol and water to evaluate its antimicrobial and antioxidative activities. We also evaluate its anti-inflammation and anticancer activity of the flower.
The result showed that most of extracts had antimicrobial activities extracts from flower. The ethyl acetate extract was the most effective among all the extracts.
Most extracts from flowers, leaves and stems showed the good antioxidative activities especially the flower. The the ethyl acetate extracts presented the best on DPPH scavenging activity and reducing power and had the highest total phenolic compounds content .
We used the ethyl alcohol and water extracts of T. procumbens to treat RAW264.7 macrophage, which induced inflammation by Lipopolysaccharide (LPS).In the NO assay, the extracts of T. procumbens flower had better inhibit
In MTT assay of SK-Hep-1, we found that extract of T. procumbens flower had the best inhibitory ability. We also found the ethyl alcohol extract of T. procumbens had the best to diminish the expression of matrix metalloproteinase (MMP)-9 and MMP-2. The ethyl alcohol extracts of flower had good anti-migration and anti-invasion ability.

並列關鍵字

Tridax procumbens Linn ； antibacterial activity ； antioxidation ； anti-inflammation ； anticancer activity

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