以啟動子轉殖細胞株篩檢調節免疫力之中藥的模式建立

Construction of Promoters Based Immunity Screening System and Its Application on the Study of Traditional Chinese Medicine Herbs

趙文婉(Wen-Wan Chao) ； 郭悅雄(Yueh-Hsiung Kuo) ； 林璧鳳(Bi-Fong Lin)

《臺灣農業化學與食品科學》 45卷4&5期 (2007/10) Pp. 193-205

https://doi.org/10.6578/TJACFS.2007.023

摘要

The purpose of this study is to establish a screening system for traditional Chinese medicine herbs, which may exert regulatory effects on immune and inflammatory responses. The scheme for the screening system is to transient transfect reporter gene luciferase with 3x-NF-κB binding site into RAW 264.7 cells to test for luciferase activity induced or suppressed by various traditional Chinese medicine herbs. The ethanol extract of these traditional Chinese medicine herbs was further fractionated with hexane, ethyl acetate (EtOAc) and water. The results showed that most of these three fractions of the traditional Chinese medicine herbs suppressed luciferase activity through NF-κB luciferase activity in LPS/IFNγ-activated RAW cells. EtOAc fraction of Taxilli chinensis, Angelica sinensis decreased in NF-κB luciferase activity (p＜0.05). EtOAc fraction of Hedyotis diffusa Willd. significantly decrease IL-6 production. Hexane fraction of Taxilli chinensis, Jujubae fructus and EtOAc fraction of Angelica sinensis, Andrographis paniculata significantly decrease NO secretion. Hexane fraction of Eucommia ulmodes Oliv., Taxilli chinensis, Jujubae fructus and EtOAc fraction of Angelica sinensis, Jujubae fructus, Glycyrrhizae radix, Astragalus membranaceus (Fisch.) Bge., Taxilli chinensis, Ligustri lusidi fructus, Andrographis paniculata, Isatidis radix significantly decrease PGE2 secretion. Therefore these herbs with anti-inflammatory effect or immunomodulatory are worthy of further study to investigate NF-κB on immune regulation.

關鍵字

短暫轉染 ； NF-κB ； RAW 264.7巨噬細胞株 ； 中草藥材

並列摘要

The purpose of this study is to establish a screening system for traditional Chinese medicine herbs, which may exert regulatory effects on immune and inflammatory responses. The scheme for the screening system is to transient transfect reporter gene luciferase with 3x-NF-κB binding site into RAW 264.7 cells to test for luciferase activity induced or suppressed by various traditional Chinese medicine herbs. The ethanol extract of these traditional Chinese medicine herbs was further fractionated with hexane, ethyl acetate (EtOAc) and water. The results showed that most of these three fractions of the traditional Chinese medicine herbs suppressed luciferase activity through NF-κB luciferase activity in LPS/IFNγ-activated RAW cells. EtOAc fraction of Taxilli chinensis, Angelica sinensis decreased in NF-κB luciferase activity (p＜0.05). EtOAc fraction of Hedyotis diffusa Willd. significantly decrease IL-6 production. Hexane fraction of Taxilli chinensis, Jujubae fructus and EtOAc fraction of Angelica sinensis, Andrographis paniculata significantly decrease NO secretion. Hexane fraction of Eucommia ulmodes Oliv., Taxilli chinensis, Jujubae fructus and EtOAc fraction of Angelica sinensis, Jujubae fructus, Glycyrrhizae radix, Astragalus membranaceus (Fisch.) Bge., Taxilli chinensis, Ligustri lusidi fructus, Andrographis paniculata, Isatidis radix significantly decrease PGE2 secretion. Therefore these herbs with anti-inflammatory effect or immunomodulatory are worthy of further study to investigate NF-κB on immune regulation.

並列關鍵字

Transfection ； NF-κB ； RAW264.7 cell ； Traditional Chinese medicine herbs