維生素B6可減緩胰島素治療糖尿病大白鼠血管併發症

Vitamin B6 Alleviates the Vascular Complications of Insulin-Treated STZ-Induced Diabetic Rats

張素瓊(Sue-Joan Chang) ； 蔣青蓉(Ching-Lung Chiang)

《中華民國營養學會雜誌》 21卷3期 (1996/08) Pp. 235-248

https://doi.org/10.6691/JCNS.199608_21(3).0002

摘要

本研究主要目的在探討維生素B6是否可减緩胰島素治療的糖尿病血管病變。以Streptozotocin (STZ)誘導糖尿病大白鼠，分別施以胰島素、維生素B6或胰島素與維生素B6同時治療。以腺 磷雙磷酸(ADP, 10μM)或凝血(Thrombin, 0.05U/mL)當致凝物，在血小板凝集儀下測定血小板凝集程度。以放射性花生四烯酸(14C-Arachidonic Acid,(上標 14)C-AA)為前驅物，利用放射性薄層層析法(Radiochromatography Scanning)及自動放射照像術(Autoradio-graphy)分析血小板生成前列凝素((上標 14)C-Thromboxane B2,(上標 14)C-TxB2)的情形，並用放射線閃爍計數器(Scintillation Counter)測量(上標 14)C-TxB2生成量。實驗結果發現，胰島素與維生素B6同時治療的實驗組，血糖值低於胰島素或維生素B6單獨治療的兩組。同時血小板凝集程度及血小板生成前列凝素程度亦有顯著降低。以上結果皆顯示，維生素B6可减緩胰島素治療糖尿病大白鼠之病情與血管幷發症的誘因，對血糖控制、防止血小板凝集、减少前列凝素生成皆比胰島素單獨治療的效果好。

關鍵字

糖尿病 ； 維生素B6 ； 血小板凝集 ； 前列凝素 ； 血管併發症 ； 去氧硝基比喃糖

並列摘要

The purpose of this study is to investigate whether vitamin B6 alleviates the vascular complications of insulin-treated streptozotocin (STZ)-induced diabetes in rats. Diabetic animals were treated with or without vitamin B6 and/or insulin. Platelet aggregation induced by ADP (10μM) or thrombin (0.05U/mL) was measured in platelet rich plasma of normal and diabetic animals. (superscript 14)C-Thrombox-ane B2 ((superscript 14)C-TxB2) production of platelets, using (superscript 14)C-Arachidonic Acid((superscript 14)-AA) as a precursor, was assayed by means of scanning radiochromatography and autoradiography. (superscript 14)C-TxB2 was quantified by scintillation counter. The results showed that vitamin B6 in conjuction with insulin treatment resulted in lower blood glucose than either vitamin B6 or insulin treatment alone. Similarly, platelet aggregation and TxB2 production in diabetics with vitamin B6 and insulin treatment were significantly decreased. These data indicated that vitamin B6 in conjunction with insulin treatment seemed to be better than vitamin B6 or insulin treatment alone in controlling blood glucose, inhibiting platelet aggregation and decreasing TxA2 production.

並列關鍵字

diabetes ； vitamin B6 ； platelet aggregation ； thromboxane B2 ； vascular complication ； streptozotocin