甘草素對STZ誘發糖尿病小鼠之抗氧化與抗發炎作用

Antioxidant and Antiinflammatory Effects of Glycyrrihizin on STZ-induced Diabetic Mice

鄭詠寧(Yong-Ning Jheng)

指導教授 ： 劉德中

中山醫學大學/健康管理學院/營養學研究所/碩士(2008年)

https://doi.org/10.6834/CSMU.2008.00073

摘要

甘草在亞洲是時常被使用的一種藥草，在中國古代即有許多醫藥典籍指出了甘草的療效，這些處方沿用至今仍歷久不衰，顯示了在中醫領域中甘草舉足輕重的地位，而在現代藥理學的領域中甘草有許多療效已被確定，其中包含了抗炎作用及抗氧化作用，這些效應大部分來自於甘草的主要組成份，甘草素。在糖尿病的病理發展過程中，氧化壓力以及發炎現象扮演重要的角色，因此，猜測給予甘草素或許可以對糖尿病小鼠產生保護機制。本研究在以STZ誘發小鼠糖尿病的動物模式下，使用Balb / cA 品系之小鼠並於其每日飲水中添加 0.5 g / l 或 1 g / l 劑量之甘草素，期間每週測量其體重變化及血糖，飼養四週後犧牲取其腎臟、脾臟及血液用以進行各項分析。研究結果顯示，飲水添加甘草素對於糖尿病小鼠有以下效能 (1)劑量依賴性的控制糖尿病小鼠的血糖、提高其體內胰臟β細胞分泌胰島素的能力。(2)降低糖尿病小鼠腎脾臟腫瘤壞死因子阿法及腎臟白介素-6的濃度，顯示甘草素可以改善糖尿病小鼠體內的發炎情況。(3)降低糖尿病小鼠腎臟組織中脂質過氧化物丙二醛濃度，使糖尿病小鼠血清之超氧化歧化酶活性回復正常。(4)劑量依賴性地降低腎臟組織中纖維網狀蛋白的濃度。實驗結果顯示甘草素可以有效控制STZ誘發糖尿病小白鼠之糖尿病理症狀、減輕其體內發炎現象及氧化壓力，這樣的結果顯示甘草素在糖尿病小鼠體內可能可以形成一保護機制而達到減緩糖尿病持續惡化的功效。

關鍵字

甘草素 ； 糖尿病 ； 細胞激素 ； 丙二醛 ； 超氧化物歧化酶腫瘤壞死因子

並列摘要

Liquorice root was well known for its antiinflammatory and antioxidative functions mainly came from the major component, glycyrrhizin. In our study, 0.5 g / l or 1 g / l of glycyrrhizin were added into drinking water of STZ-induced diabetic mice. After 4- week treatment, mice were sacrificed and then serum samples , homogenized kidney and spleen tissues were analyzed. The data showed increased serum insulin level and decreased blood glucose in diabetic mice after drinking glycyrrhizin water (p<0.05). TNF-α level in spleen tissue and IL-6 level in kidney tissue were decreased in diabetic mice with glycyrrhizin intake; however, only 1 g / l glycyrrhizin had significant effect (p < 0.05). MDA level in kidney tissue of diabetic mice were reduced dose-dependently with glycyrrhizin intake (p < 0.05), SOD enzyme activity in diabetic mice recovered to normal status after glycyrrhizin intake (p<0.05). These results indicated the oxidative stress were decreased in the diabetic mice with glycyrrhizin intake. Fibronectin level of homogenized kidney tissue decreased dose-dependently in diabetic mice treated with glycyrrhizin (p<0.05). Our study supports that the antiinflammatory and antioxidative effects of glycyrrhizin on STZ-induced diabetic mice. It also shows that glycyrrhizin may have the ability to prevent kidney from fibronectin accumulation.

並列關鍵字

glycyrrhizin ； diabetes mellitus ； MDA ； IL-6

參考文獻

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