麩醯胺補充對STZ誘導糖尿病鼠發炎反應與氧化壓力之影響

Effects of glutamine supplementation on inflammation and oxidative stress in STZ-induced diabetic rodents

蔡佩璇(Pei-Hsuan Tsai)

指導教授 ： 葉松鈴

臺北醫學大學/公共衛生暨營養學院/保健營養學研究所/博士(2011年)

https://doi.org/10.6831/TMU.2011.00014

摘要

為了探討麩醯胺對糖尿病引發之發炎反應與氧化壓力之影響，我們建立了第一型(Type 1 diabetes mellitus, T1DM)與第二型(T2DM)的糖尿病動物模式。將實驗動物分成三組，分別為控制組(normal control, NC)、糖尿病組(diabetes without glutamine, DM)、糖尿病餵食麩醯胺組(diabetes with glutamine, DM-Gln)，結果顯示，T1DM小鼠血漿中葡萄糖、果糖胺與黏著分子皆高於正常組，DM組其組織中nitrotyrosine濃度與myeloperoxidase (MPO)活性高於NC組，而glutathione redox ratio(GSH/GSSG)比值低於NC組，但DM-Gln與NC組則無顯著差異，可知補充Gln可降低T1DM發炎反應與氧化壓力。第二部分結果顯示，T2DM給予Gln後可以降低發炎相關介質包括：interleukin (IL)-6、IL-23、monocyte chemotactic protein (MCP)-1、receptor of advanced glycation end products (RAGEs)、glutamine fructose-6- phosphate amidotransferase (GFAT)與thioredoxin interacting protein (TXNIP)等基因之表現，Gln也可改善血中抗氧化酵素活性與總抗氧化力，且降低腎臟中nitrotyrosine濃度與TXNIP基因之表現量。由以上結果可知，不論T1DM或T2DM補充麩醯胺，可增加體內抗氧化能力進而降低氧化壓力與發炎反應，減少器官氧化傷害。

關鍵字

麩醯胺、糖尿病、發炎介質、氧化壓力

並列摘要

This study investigated the effects of glutamine (Gln) supplementation on inflammation and oxidative stress in diabetic animals. There were 3 groups in this experiment, including normal control group (NC), diabetic group without Gln (DM) and diabetes with Gln supplementation (DM-Gln). Results in T1DM showed that levels of plasma glucose, fructosamine and adhesion molecules were significantly higher in the diabetic groups than those in the NC group. The DM group had higher organ nitrotyrosine concentrations as well as myeloperoxidase activities whereas ratio of reduced and oxidized glutathione (GSH/GSSG) was lower than those of the NC group. However, these parameters did not differ between the DM-Gln and the NC group. Results in T2DM showed that, compared with the DM group, gene expressions of inflammatory mediators including interleukin (IL)-6, IL-23, monocyte chemotactic protein (MCP)-1, receptor of advanced glycation end products (RAGEs), and glutamine fructose-6-phosphate amidotransferase (GFAT), thioredoxin interacting protein (TXNIP) were lower than those in DM-Gln group. Also, Gln supplementation enhanced GSH/GSSG and total antioxidant capacity and normalized antioxidant enzyme activities. The nitrotyrosine concentration and TXNIP gene expression in kidney tissue were lower in DM-Gln than those in the DM group. These results suggest that supplemental dietary Gln increased antioxidant potential and may consequently decrease inflammatory mediator expression and oxidative damage to organs in rodents with T1DM or T2DM.

並列關鍵字

glutamine, diabetes mellitus, inflammatory mediator, oxidative stress

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