洛神花水萃取物及多酚對STZ誘導糖尿病大白鼠腎病變和脂質代謝之影響

The effect of liquid extracts and polyphenol extracts from Hibiscus Sabdariffa Linnaeus on nephropathy and lipid metabolism in STZ-induced diabetic rats

陳宇欣(Yu-Hsin Chen)

指導教授 ： 李彗禎 副教授

中山醫學大學/醫學院/生化暨生物科技研究所/碩士(2006年)

https://doi.org/10.6834/CSMU.2006.00086

摘要

糖尿病極易引發心血管疾病及腎衰竭等併發症而致死，這是由於糖尿病患者因長期處於高血糖環境下，體內產生較多的自由基，並因體內抗氧化防禦系統能力的降低，使得糖尿病患者體內的氧化壓力引發脂質代謝不良及腎臟損傷所致。
本研究利用streptozotocin (STZ ) 65mg/kg，以腹腔注射來誘發Sprague-Dawley (SD) rats糖尿病的發生後，每日依體重給予洛神花水層萃取物 (HSE)100mg/kg、400mg/kg及洛神花多酚 (HPE) 100mg/kg、200mg/kg。在血清生化值的分析上，並沒有觀察到對血糖的抑制效果，而在血清白蛋白、三酸甘油脂、總膽固醇及低密度脂肪酸的改善，可以發現良好的效果。因此以TBARS assay加以分析脂質過氧化的程度，可觀察到給予HSE、HPE可以有效的降低脂質過氧化的程度。另外，在腎臟組織切片可以發現，以STZ誘發糖尿病，可以觀察到腎小管的細胞明顯出現了許多的透明水腫的變化，這可能是由於葡萄糖造成滲透壓的改變，使細胞受到損傷所致，給予HSE及HPE可以改善此現象。進一步分析腎臟蛋白表現，可以觀察到HSE、HPE經由增加腎臟Akt磷酸化，使Bad和14-3-3γ結合增加；以EMSA分析，發現NF-κB的轉錄活性由於HSE和HPE的作用而增加，因而促進腎臟的cell survival。由此我們推測HSE及HPE對於脂質代謝的改善及腎臟的保護有其效果。

關鍵字

糖尿病 ； 多酚 ； 腎病變 ； 脂質代謝

並列摘要

The major mortality of diabetes mellitus (DM) was usually caused of the complications, such as the cardiovascular diseases and renal failure. This result could be concluded that under the circumstance of high blood sugar for a long time, abundant free radicals could be produced in diabetes patients; and furthermore, the ability of anti-oxidation could be reduced. The oxidative stress could cause macromolecules oxidative and further damage the organ, such as kidney and cardiovascular system.
In this study, streptozotocin (STZ) was administrated to induce SD rats type I DM. After the induction completed, 100mg/kg、400mg/kg Hibiscus sabdariffa extracts (HSE) or 100mg/kg、200mg/kg Hibiscus sabdariffa polyphenols (HPE) were fed by oral tube to observe the effects on DM rats. In serum data, we did not observe the restrain result to the blood glucose. However, either HSE or HPE treated groups, the levels of serum albumin, triglyceride, total cholesterol and low density lipoprotein were improved. In TBARs assay, HSE and HPE also possessed the ability to reduce lipid peroxidation in liver. Additionally, in kidney section, we observed the hydropic change caused of the glucose, it would make the cell damage. When HSE or HPE pretreated, this phenomena was improved. Further, in kidney protein lysate, we found HSE or HPE could protect the kidney via increasing Akt expression to acivate Bad and 14-3-3γ expressions, and regulate the transcription factor NF-κB to promote kidney cell survive. We assumed that via reducing oxidative stress, HSE and HPE possessed the ability to improve the high glucose induced abnormal lipid metabolism and protect kidney damage.

並列關鍵字

Hibiscus Sabdariffa Linnaeus ； polyphenol ； nephropathy ； STZ

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