膳食添加紅心芭樂對於 STZ 誘發糖尿病小白鼠病情改善之研究

Effects of Psidium guajava L. supplementation on STZ-induced Diabetes mice

李佩瀅(Pei-Ying Lee)

指導教授 ： 趙哲毅

亞洲大學/健康學院/保健營養生技學系碩士班/碩士(2011年)

摘要

糖尿病是台灣地區重要的慢性病之一，且高血糖所誘發的氧化傷害是造成糖尿病患者發生慢性併發症之主因。番石榴 (Psidium guajava L.) 為熱帶水果的一種，屬於桃金孃科（Mytaceae），是台灣重要的經濟作物之一。本研究分成兩部份，第一部份為探討紅心芭樂之乙醇及乙酸乙酯萃取物，其抗氧化及抗發炎能力測定。結果顯示，紅心芭樂乙醇萃取物之抗氧化及抗發炎能力皆顯著高於乙酸乙酯萃取物。第二部份為探討膳食添加紅心芭樂對於以 streptozotocin (STZ) 誘發糖尿病 Balb/c小鼠的保護作用。實驗組別分為正常組 (N)、糖尿病組 (DM)、糖尿病添加1% (L)、2% (M)、5%(H) 紅心芭樂組、5% 紅心芭樂合併抗糖尿病藥 Rosiglitazone 組 (HR) 及 Rosiglitazone組 (R) 等七組，餵食八週後採斷頭法犧牲。結果發現，膳食添加紅心芭樂的組別與糖尿病組相比，其血糖控制、胰島素阻抗、肌酸酐、尿素氮、三酸甘油酯、非酯化游離脂肪酸、膽固醇及 C-反應蛋白皆有顯著改善。並可藉由活化 PPAR 基因表現，進而抑制 iNOS 及 NF-B 等蛋白質之表現，亦可增加 GPx3、ACO 蛋白質之表現量。結果顯示，膳食添加紅心芭樂可減緩糖尿病的病症，具有保護作用及開發為保健食品之潛力。

關鍵字

紅心芭樂 ； 糖尿病 ； STZ ； 抗氧化 ； 抗發炎

並列摘要

Diabetes was one of the major chronic diseases in Taiwan. Hyperglycemia-induced oxidative stress may play an important role in the development of chronic complications in diabetes mellitus. Guava (Psidium guajava L.) is a tropical fruit, which belongs to the family Myrtaceae, it is one of the most important economic fruit in Taiwan. Leaves and fruits of guava have been reported to have anti-diarrheal, hypoglycemic, lipid lowering, anti-bacterial and anti-oxidative activities. This study was divided into two parts, first part is to evaluate the ethanol and ethyl acetate extract of guava which anti-oxidative and anti-inflammatory ability. The second part of study is to observe the effects of guava supplementation in STZ-induced type 2 diabetes mice. All Balb/c mice were classified into seven groups as follows: normal group (N), diabetes group (DM), guava supplement groups (L, M, H), 5% guava and rosiglitazone supplement group (HR), and rosiglitazone group (R). In vitro study showed that the ethanol extract of guava has better anti-oxidative and anti-inflammatory activities. In vivo study showed that guava supplement groups could significantly improved the blood sugar, insulin resistance, creatinine, blood urea nitrogen, trigylcerides, cholesterol, non-esterified free fatty acids, C-reactive protein than diabetes group. As a result of PPARactivation lead to reduce the iNOS and NF-B protein expression and increase the GPx3 and ACO protein expression. These results indicated that guava supplement could attenuated the diabetic condition and possessed the pontential to developed the healthy foods.

並列關鍵字

Psidium guajava L. ； Diabetes ； STZ ； anti-oxidation ； anti-inflammation

參考文獻

許惠恒 (2006)。糖尿病合併血脂異常之治療。中華民國糖尿病衛教學會會訊。

郭豐吉 (2008)。糖尿病腎病變-蛋白尿的含義及治療。中華民國糖尿病衛教學會會訊。

蔡明綺，樊謙騰，陳鏡潭 (2007)。番石榴 (Psidium guajava Linn.) 乾燥葉、葉芯、果實水萃液及日本市售番石榴葉飲料之α-葡萄糖苷酶、α-澱粉酶抑制活性及活性氧清除能力。台灣農業化學與食品科學，45(2):76-83。

卓亭均 (2008)，抗氧化複方對 streptozotocin 誘發之糖尿病大鼠致血栓危險性之探討。靜宜大學食品營養學系碩士論文。

范嘉玲 (2005)。維生素C與維生素E之補充對抑制第2型糖尿病患活化血小板生成之探討。靜宜大學食品營養學系碩士論文。