β-胡蘿蔔素在有或無膽固醇攝取下對大白鼠脂質代謝的影響

Effects of β-Carotene on Lipid Metabolism in Rats Fed with or without Cholesterol

施純光(Chun-Kuang Shih) ； 鄭心嫻(Hsing-Hsien Cheng) ； 謝明哲(Ming-Jer Shieh)

《中華民國營養學會雜誌》 22卷3期 (1997/08) Pp. 287-301

https://doi.org/10.6691/JCNS.199708_22(3).0004

摘要

本研究之目的是探討β-胡蘿蔔素在有或無膽固醇攝取下對大白鼠脂質代謝的影響。將40隻Wistar品系雄性大白鼠（重約250g）隨機分成4組，以1％膽固醇的給予或不給予，及0.2%β-胡蘿蔔素的給予或不給予，作2×2 factorial實驗設計。各組均於第3週及第6週分別宰殺半數大白鼠，分析其血清及肝臟中總膽固醇(TC)、三酸甘油酯(TG)濃度，血清並分析其中高密度脂蛋白膽固醇(HDL-C)及低密度脂蛋白膽固醇(LDL-C)濃度，此外利用HPLC分析血清及肝臟中視網醇及β-胡蘿蔔素濃度。實驗結果顯示，當飼料中添加膽固醇時，在第3週即造成肝臟TC濃度顯著升高，血清TC濃度則在第6週時上升，且以未添加β-胡蘿蔔素之膽固醇組上升最高。第3週時與無膽固醇組相比，膽固醇組之血清HDL-C濃度較低，而LDL-C濃度無顯著差異；第6週時，未添加β-胡蘿蔔素之膽固醇組HDL-C濃度仍低，而LDL-C濃度顯著升高，添加β-胡蘿蔔素之膽固醇組則無此現象，且HDL-C濃度顯著上升。膽固醇的給予使肝臟TG在第3週時即顯著升高，血清TG濃度則無論在第3週或第6週時均與無膽固醇組間無顯著差異；β-胡蘿蔔素的給予在第3週時使肝臟TG濃度降低，並使血清TG濃度升高，在第6週時則與未給予β-胡蘿蔔素組間無顯著差異。攝取膽固醇使肝臟視網醇濃度下降，攝取β-胡蘿蔔素則使肝臟視網醇濃度上升，但不影響血清視網醇濃度。血清β-胡蘿蔔素濃度因膽固醇之攝取而下降，肝臟β-胡蘿蔔素濃度則不受膽固醇攝取之影響。

關鍵字

β-胡蘿蔔素 ； 膽固醇 ； 大白鼠 ； 脂質代謝

並列摘要

The purpose of this study was to investigate the effect of β-carotene on lipid metabolism in rats fed with or without cholesterol. Forty male adult Wistar rats were randomly divided into four groups in a 2×2 factorial design according to 1% cholesterol (with or without) and 0.2% β-carotene (with or without). Half of rats of each group were killed after three- or six-week feeding, then liver and artery blood were collected. Items analyzed included serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), liver total cholesterol, and liver triglyceride. Retinol and β-carotene in serum and liver were analyzed by HPLC. The results showed that cholesterol-fed groups had significantly higher liver, total cholesterol concentrations after three-week feeding and higher serum total cholesterol concentrations after six-week feeding, especially in the non-β-carotene-fed group. Cholesterol-fed groups had lower serum HDL-C concentrations after three-week feeding, but there was no difference in the LDL-C concentration. After six-week feeding, HDL-C concentration was still lower in rats fed cholesterol but no β-carotene whereas LDL-C concentration rose significantly. Rats fed cholesterol and β-carotene had no such condition and the HDL-C concentration rose significantly. Cholesterol feeding made the liver TG concentration higher after three-week feeding, but there was no difference in the serum TG concentration after either three- or six-week feeding. β-Carotene feeding showed lower liver TG concentration and higher serum TG concentration after three- week feeding, but no difference in serum TG concentration between β-carotene-fed and non-β-carotene-fed groups after six-week feeding. Cholesterol feeding made the liver retinol concentration lower whereas β-carotene feeding made the liver retinol concentration higher but had no effect on the serum retinol concentration. The serum β-carotene concentration was decreased whereas the liver β-carotene concentration was not affected due to cholesterol feeding.

並列關鍵字

β-carotene ； cholesterol ； rats ； lipid metabolism