Comparison of Oocyte Morphology with Serum and Follicular Fluid FSH, LH, PRL and E2 in Women Undergoing in Vitro Fertilization

接受體外受孕婦女的血清及卵泡液中之性激素濃度與卵子成熟度之比較

師明哲(Min-Tsir Shih) ； 余慕賢(M. S. Yu) ； 許志學(C. H. Hsu) ； 朱堂元(T. Y. Chu) ； 李家琨(C. K. Lee)

《Journal of Medical Sciences》 8卷6期 (1988/01) Pp. 367-375

摘要

測量接受本院體外受孕計劃的七十九位病人血清中及卵泡液中之卵泡促進激素，黃體促進激素、催乳素和雌二醇濃度。七十九位病人共抽取三○七個卵泡，發現二三五個卵子，其中一六六個卵子受孕成功，一二五個分裂，有八位病人顯示生化妊娠，二位病人成功妊娠及生產。卵子的成熟度依觀察分四組，依次為萎縮、不成熟、成熱第一級，和成熱第二級，分析卵子的成熟度和血清中及卵泡液中激素濃度比較，其中血清及卵泡液中激素濃度越高者，卵子越成熟，同時合併之不成熟卵也愈多。血清及卵泡液中之雌二醇值呈現與卵子成熟、受孕、分裂及妊娠相當有關。而卵泡促進激素可增加雌二醇之產生，然催乳素及黃體促進激素則不然。

關鍵字

卵子成熟度 ； 卵泡液

並列摘要

The Serum and follicular fluid concentrations of Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), Prolactine (PRL) and Estradiol (E2) were measured in 79 cases undergoing In-Vitro Fertilization (IVF). In our hospital-based IVF program, 307 follicles were followed, 225 eggs recovered, 166 eggs fertilized, 125 eggs cleft, and 8 biochemical pregnancies and 2 full term babies were achieved. The maturity levels of the oocytes were divided into 4 groups: immature, mature (stage I), mature (stage II), and atretic. The morphology and maturity of the oocytes were analyzed and compared with the hormone levels in serum and follicular fluid. More mature oocytes were produced with higher levels of FSH, LH, PRL and E2 in serum and follicular fluid. At the same time, more immature oocytes were produced as well. (FSH: R=0.78, P＜0.01; LH: R=0.53, P＜0.01; PRL: R=0.53, P＜0.01). The estradiol levels in both serum and follicular fluid showed good correlation with oocyte maturity, fertility, cleavage and with pregnancy rate (p＜0.01). FSH enriched follicular E2 production (R=0.69, P＜0.01), and the releasing of FSH were accompanied by a marked increase of circulating E2, whereas similar responses were not observed with varying levels of PRL and LH.

並列關鍵字

Oocyte Maturity ； Follicular fluid