Effects of Hemodialysis on Lymphocyte Subpopulations in Uremic Patients

血液透析對尿毒症患者周邊血液中淋巴球亞型之影響

周貝倫(Bey-Luen Chou) ； 吳三江(San-Giang Wu) ； 李文俊(Wen-June Lee) ； 蕭文衍(Wen-Yen Hsiao) ； 王丹江(Dan-Jiang Wang)

《Journal of Medical Sciences》 13卷4期 (1993/02) Pp. 277-284

摘要

Cellular immune function has been demonstrated to be altered in many diseases. By using flow cytometry (FACScan), we analyzed the subpulations of peripheral blood lymphocytes from 15 patients with uremia on chronic hemodialysis. The results indicated significant lymphopenia (p＜0.05) with diminished pan T cell (CD3) (p＜0.05) and increased granulocytes (p＜0.05) in uremic patinets. The percentage of T suppressor cell (CDs) increased (p＜0.05) and this change resulted a decrease (p＜0.05) in the mean ratio of CD4/CDs. However, the proportion of T helper cell (CD4), B cell (CDl9) and monocyte were not changed to compare the control subjects. When absolute counts of lyphocyte subpopulations were determined, more striking differences were found between hemodialyzed patients and control subjects. ANOYA analysis showed a reduced numbers of pan T cell (CD3) (p ＜ 0.05) in pre-hemodialyzed group as compared with normal subjects and posthemodialyzed group, while the T suppressor cell (COs) (p ＜ 0.05), T activated cell (CD3DR) (p ＜ 0.01) and natural killer cell (CDl6) (p ＜ 0.05) were increased. We concluded that the derangement of immunoregulations is definitely present in uremic patients and is not completely restored after hemodialysis. To elucidate the mechanism of this impaired immune response, the further study of the differentiation of discrete lymphocyte subpopulations in the impairment of cellular immunity in uremia is needed.

關鍵字

淋巴球亞型 ； 尿毒症 ； 血液透析

並列摘要

Cellular immune function has been demonstrated to be altered in many diseases. By using flow cytometry (FACScan), we analyzed the subpulations of peripheral blood lymphocytes from 15 patients with uremia on chronic hemodialysis. The results indicated significant lymphopenia (p＜0.05) with diminished pan T cell (CD3) (p＜0.05) and increased granulocytes (p＜0.05) in uremic patinets. The percentage of T suppressor cell (CDs) increased (p＜0.05) and this change resulted a decrease (p＜0.05) in the mean ratio of CD4/CDs. However, the proportion of T helper cell (CD4), B cell (CDl9) and monocyte were not changed to compare the control subjects. When absolute counts of lyphocyte subpopulations were determined, more striking differences were found between hemodialyzed patients and control subjects. ANOYA analysis showed a reduced numbers of pan T cell (CD3) (p ＜ 0.05) in pre-hemodialyzed group as compared with normal subjects and posthemodialyzed group, while the T suppressor cell (COs) (p ＜ 0.05), T activated cell (CD3DR) (p ＜ 0.01) and natural killer cell (CDl6) (p ＜ 0.05) were increased. We concluded that the derangement of immunoregulations is definitely present in uremic patients and is not completely restored after hemodialysis. To elucidate the mechanism of this impaired immune response, the further study of the differentiation of discrete lymphocyte subpopulations in the impairment of cellular immunity in uremia is needed.

並列關鍵字

Lymphocyte subpopulations ； Uremia ； Hemodialysis