Fistula needle set is used for haemodialysis. This report covers the development work of A.V. Fistula cannula with intention to manufacture it according to Good Manufacturing Practice. Commonly considered biological safe polymers were evaluated in terms of their compatibility with blood. Medical grade soft and rigid polyvinyl chloride, polystyrene, and polyethylene were selected to produce various parts for the cannula. Manufacturing machinery and method, test instrument, test method, and specifications were in details in this report.