Expression of Vascular Endothelial Growth Factor in Hepatocellular Carcinoma Cell Lines and Patients

肝癌細胞株及肝癌病人血管內皮細胞生長因子之表現

柯成國(Chen-Guo Ker) ； 陳鴻耀(Hong-Yaw Chen) ； 阮仲洲(Chon-Chou Juan) ； 曾建森(Chien-Sen Tseng) ； 孫盟舜(Meng-Shun Sun) ； 羅海韻(Hoi-Wan Lo) ； 施登富(Teng-Fuh Shih) ； 羅偉菁(Wei-Ching Lo)

《Formosan Journal of Surgery》 39卷3期 (2006/06) Pp. 126-132

https://doi.org/10.29833/FJS.200606.0003

摘要

血管內皮細胞生長因子(Vascular Endothelial Growth Factor, VEGF)是肝癌血管成長過程中之重要因素之一。本研究將探討不同肝癌細胞株及臨床肝癌病人血清中VEGF濃度，分析其與細胞分化程度及年齡群有何不同。
從肝癌細胞株Hep3B（分化良好）、J5（分化中度）及Mahlavu及SK-Hep-1（分化差）共四種以供實驗，從其培養液中測其分泌VEGF之濃度。另外從43位男性肝癌病例中皆經手術切除且病理證實肝癌者，依其分化程度及年齡群而分析。
從這四種肝癌細胞株之分泌VEGF分為HeP3B為332.4±49.4、J5為204.4±49.4、Mahlavu為111.7±284.4及SK-Hep-1為54.01±6.1，可見其分化差者其VEGF濃度亦低。在臨床病例其分化良好、中度及差者分別為423.4±115.3、356.7±106.5及116±45.6ng/dl，所以分化差者其濃度亦低。年齡在40歲以下者其VEGF濃度較高。
結論是肝癌細胞分化差者其VEGF濃度亦較低，所以分化差之肝癌其預後較差不單只是VEGF因素而已。如果考慮到臨床上之應用抗腫瘤新生血管因素，應在分化良好之早期肝癌為佳。

並列摘要

Objective: The vascular endothelial growth factor (VEGF) is thought to take an important role in tumor angiogenesis. The present study examined VEGF expression in the liver cancer cell lines and human liver cancer of various differetiated degrees.
Methods: Four human hepatocellular carcinoma cell lines with varying degrees of differentiation, namely: Hep 3B (well differentiated), J5 (moderately differentiated), and Mahlavu plus SK-Hep-I (poorly differentiated) were used. For measuring VEGF, culture cells (5-7.5×10^5/ml) were seeded onto 100mm dishes for 24 hours. The amount of secreted VEGF in the culture medium of the cells was measured by the enzymelinked immunosorbent assay (ELISA) method. Forty-three male patients of hepatocellular carcinoma (HCC) were encountered for measuring the serum VEGF level with relationship with cancer cell differentiation.
Results: In all four cell lines, VEGF was secreted to the culture supernatants. After 24-hour culture, the VEGF secretion levels were as follows: 332.4±88.1 in Hep 3B; 204.4±49.4 in J5; 111.7±28.4 in Mahlavu; 54.01±6.1 in SK-Hep-I. VEGF secretion in SK-Hep-I was the lowest. In the HCC patients, the serum VEGF level were 423.4±115.5, 356.7±106.5, and 116±45.6 ng/dl for different cancer cell types: well, moderately and poor differentiated groups respectively. In this study, the highest concentration of VEGF was obtained in the well differentiated liver cancer cells.
Conclusions: The expression of VEGF was higher in the well or moderately differentiated liver cancer cell in vivo or in vitro study. Clinical biological behavior of poorly differentiated liver cancer may not have resulted from VEGF alone. This study indicats that the high concentration of VEGF has clinical meaning in well differentiated HCC which usually is not presented in the late stage of hepatocarcinogenesis. Therefore, if we try to apply any anti-angiognetic agents to treat HCC, we suggest that the patients are well differentiated HCC.

並列關鍵字

vascular endothelial growth factor ； hepatocellular carcinoma ； cell differentiation