Immunohistochemical Study of Hepatocyte Growth Factor in Non-Small-Cell Lung Cancers

非小細胞癌之肝細胞生長因子免疫組織化學研究

洪仁宇(Jen-Yu Hung) ； 侯俊君(Jiunn-Jiun Hou) ； 許德宏(Te-Hung Hsu) ； 陳俊男(Jiuan-Nan Chen) ； 王東衡(Tung-Heng Wang) ； 鍾飲文(Inn-Wen Chong) ； 黃吉志(Jhi-Jhu Hwang) ； 高英隆(Eing-Long Kao) ； 黃明賢(Ming-Shyan Huang)

《胸腔醫學》 15卷3期 (2000/09) Pp. 126-133

摘要

肝細胞生長因子被認為是一種多功能性之生長因子具有多種生物學功能作用。但是至今肝細胞生長因子在人體內的生理功能機轉及所具有的角色尚未十分明白。我們用免疫組織化學方法研究肝細胞生長因子在非小細胞肺癌之表現。二十位非小細胞肺癌病例進入比研究，針對這二十個肺癌腫瘤標本，進行肝細胞生長因子之免疫組織化學染色。結果有十六位病例免疫組織化學染色呈現陽性反應，非小細胞肺癌細胞的細胞質呈現肝細胞生長因子免疫組織化學陽性反應，肺癌組織間質及纖維細胞亦有弱的肝細胞生長因子之免疫組織化學陽性反應，組織的小血管壁呈現強度的肝細胞生長因子之免疫組織化學陽性反應。由我們的研究結果，顯示肝細胞生長因子可能在非小細胞肺癌細胞的繁殖生長上具有重要的角色，可能在非小細胞肺癌的腫瘤血管增生上亦具有其作用功能。今後需要作更進一步深入研究。

關鍵字

肝細胞生長因子 ； 免疫組織化學 ； 非小細胞肺癌

並列摘要

Hepatocyte growth factor (HGF) is a multifunctional growth factor with a broad spectrum of biological effects. However, the physiological role of HGF in vivo remains largely unknown. We studied HGF in patients to investigate its clinical significance in non-small-cell lung cancers (NSCLC). Twenty patients with NSCLC were studied. HGF immunohistochemical staining was used to study twenty NSCLC tumor tissue specimens. Sixteen of 20 NSCLC tumors were positive for the anti-HGF immunohistochemical stain, and NSCLC cells showed a diffused cytoplasm immunoreactivity for HGF. The stroma of all specimens showed variable and weak fibroblastic immunoactivity for HGF, but there was strong immunoactivity over the small vascular walls. In conclusion, our results suggest that HGF may play an important role in the growth and behavior of NSCLC. Further investigation may be required.

並列關鍵字

hepatocyte growth factor ； HGF ； Immunohistochemistry ； non-small-cell lung cancer