糙薏仁對餵與高脂高膽固醇飲食倉鼠血液脂質及肝臟損傷指標的影響

Effects of dehulled adlay on serum lipid profiles and hepatic injury markers in hamster fed with a high-fat-high-cholesterol diet

劉怡君(Yi-Chun Liu) ； 莎西(Sassy Bhawamai) ； 陳裕星(Yu-Hsin Chen) ； 陳玉華(Yue-Hwa Chen)

《台灣營養學會雜誌》 45卷1期 (2021/03) Pp. 26-34

https://doi.org/10.6691/NSJ.202103_45(1).0003

摘要

高脂及高膽固醇飲食的攝取一般皆伴隨高脂血症、肝臟脂肪堆積與損傷的發生。糙薏仁是國人常攝取的主食穀物。本研究主要探討糙薏仁（dehulled adlay, DA）對於因高脂高膽固醇飲食（high fat high cholesterol diet, HFHCD）所誘導之的血脂、肝臟脂肪堆積與損傷的影響。雄性Syrian hamster倉鼠隨機分為5組並餵與8週不同飲食，包括基礎飲食、HFHCD、HFHCD合併10,20與40%冷凍乾燥之DA粉末，動物犧牲後採集血液、肝臟及糞便進行分析。結果顯示高脂高膽固醇飲食所誘導增加的血膽固醇、低密度脂蛋白膽固醇（LDL-C）與脂質過氧化指標（malondialdehyde, MDA）濃度、動脈粥狀硬化係數（atherogenic coefficient, AC）等皆因為20及40%糙薏仁的介入而顯著降低；雖然糙薏仁的介入使肝臟膽固醇有隨之累積增加現象，但是肝臟aspartate aminotransferase（AST）、alanine aminotransferase（ALT）、matrix metalloproteinase（MMP）-2及MMP-9活性等損傷指標卻都顯著下降。總言之，飲食中20與40% DA可降低餵與高脂高膽固醇飲食倉鼠血中膽固醇、LDL-C、AST、ALT濃度並抑制肝中MMP-2及MMP-9活性，因而對高脂血症及肝損傷具有保護的作用。

關鍵字

高脂高膽固醇飲食 ； 高脂血 ； 肝臟脂質堆積 ； 肝臟損傷指標 ； 糙薏仁

並列摘要

Consumption of high fat and high cholesterol diet is always associated with hyperlipidemia, increased hepatic lipid accumulation, oxidative stress, and liver injury. Dehulled adlay (DA) is one of the staple foods consumed by Taiwanese people. This study is aimed at investigating the effects of DA on hyperlipidemia, hepatic lipid accumulation and injury in animals fed with a high-fat high-cholesterol diet (HFHCD). Male Syrian hamsters were randomly assigned into 5 groups and fed with different diets, control, HFHCD, HFHCD with 10, 20, and 40% freezed-dried DA, for 8 weeks. The animals were sacrificed, and blood, liver, and fecal samples were collected for analysis. The results showed that 20 and 40% DA significantly reduced serum total cholesterol (TC), low density lipoprotein- cholesterol (LDL-C) and malondialdehyde (MDA) induced by HFHCD, and such effects were associated with decreased atherogenic coefficient (AC). Besides, reduction of hepatic injury markers, aspartate aminotransferase (AST), alanine aminotransferase (ALT), matrix metalloproteinase (MMP)-2, and MMP-9 activities were also observed in 20 and 40% DA groups, although hepatic TC accumulation was increased. In conclusion, 20 and 40% DA has protective effects on hyperlipidemia and hepatic injury by decreasing serum TC, LDL-C, AST, ALT concentrations, and hepatic MMP-2 and MMP-9 activities in hamster fed with a HFHCD.

並列關鍵字

HFHCD ； hyperlipidemia ； hepatic lipid accumulation ； liver injury marker ； dehulled adlay