Investigation on the Production Kinetics of Lovastatin by Aspergillus terreus in a 5 L Fermentor

探討在5升醱酵槽中真菌Aspergillus terreus生產lovastatin之動力學

賴龍山(Long-Shan T. Lai) ； 王德期(Te-Chi Wang) ； 鄭宗堯(Tsung-Yao Cheng)

《Fungal Science》 19卷3&4期 (2004/12) Pp. 97-107

https://doi.org/10.7099/FS.200412.0097

摘要

在真菌Aspergillus terreus之5升醱酵槽操作中，若供應相對較低的液中溶氧(DO)幾乎會停止其二次代謝產物lovastatin之生產；與搖瓶實驗相較，當控制DO 20%時，產物生產大幅提高了38%，此時菌絲量降低25%而糖源利用率則提高18%。若自生產期後的48小時起，將pH值控制在5.5、6.5或7.5，它雖顯著減少細胞產量但卻無助於產物之生合成；本文並發現pH-DO間交互作用會影響此醱酵系統之回應。簡言之，真菌A. terreus二次代謝產物lovastatin之增產是受其培養環境因子之控制與因子間之互相影響，它也同時造成菌絲增長形態之改變。

關鍵字

Aspergillus terreus ； lovastatin ； 溶氧 ； 醱酵槽操作

並列摘要

Using a 5 L fermentor, a comparatively low supplement of dissolved O2 (DO) almost stopped performing lovastatin synthesis using Aspergillus terreus. With the DO controlled at 20%, lovastatin production enhanced by 38% whereas biomass production decreased by 25% and sugar utilization increased by 18%, as compared with the shake-flask culture. However, manipulating the broth pH at 5.5, 6.5 or 7.5 starting from 48 h gave no benefit to product formation although biomass production was reduced largely. A pH-DO interaction was also found here, which significantly affected the fermentation response. In the study, we concluded that the enhancement of product formation closely depended upon the controls of culturing environments; meanwhile, the fungal morphology was also influenced by the fermmentation parameters and the interactions.

並列關鍵字

Aspergillus terreus ； dissolved O2 ； fermentor operations ； lovastatin