Purification and Characterization of Xylanase from Xylaria regalis

炭角菌木聚糖酵素之純化及其生化特性之探討

魏玎玲(Ding-Ling Wei) ； 周慧琴(Hui-Chin Chou) ； 鄭閔魁(Min-Kuei Cheng) ； 張勝祺(Shenq-Chyi Chang)

《Fungal Science》 20卷1&2期 (2005/06) Pp. 53-59

https://doi.org/10.7099/FS.200506.0053

摘要

炭角菌(Xylaria regalis)產生之木聚糖酵素經由離子交換樹脂管柱、疏水性管柱及分子篩膠體管柱之純化，得到最終產物活性提高10.98倍，回收率為34.6%。經由SDS-膠體電泳分析得知此酵素分子量為30.8kDa；此外，本酵素最適反應溫度50℃，最適反應pH值5.5。金屬離子Cu(上標 2+)、Hg(上標 2+)及化合物EDTA或β-mercaptoethanol會抑制此酵素之活性。

關鍵字

木聚糖酵素 ； 炭角菌

並列摘要

An extracellular xylanase was purified to homogeneity from the culture filtrate of ”Xylaria regalis”. The xylanase was purified 10.98 fold, giving a 34.6% yield. The crude enzyme was subjected to a series of the purification procedures, including QAE Sephadex A-25 anion exchange column, hydrophobic interaction phenyl 650M column and Sephadex G-75 gel filtration column chromatography, respectively. The molecular mass of the purified xylanase estimated by SDS-PAGE was approximately 30.8 kDa. The optimal temperature of the enzyme activity was 50℃. The optimal pH of xylanase activity was 5.5 and the enzyme appeared to be stable over the pH range from 5-9. The enzyme activity was inhibited by Cu(superscript 2+), Hg(superscript 2+), EDTA, and β-mercaptoethanol.

並列關鍵字

Xylanase ； Xylaria regalis