- 期刊
Evaluation of a High-speed Centrifuge with Rapid Preparation of Plasma for Coagulation Testing to Improve Turnaround Time
以快速離心處理血液凝固檢體縮短血液檢驗報告時間之評估
摘要
檢驗報告完成時間(TAT)經常被用來作為臨床實驗室服務品質良窳最顯著的績效指標。本研究的目的是評估一種新的凝血測試檢體快速離心法，以提供更快速的檢驗報告並改善TAT。本研究依序從10個自願者收集了6管2毫升血液，分別依序在傳統離心機上以1,500g離心15分鐘，在高速離心機以4,000g、5,000g、6,000g、7,000g、8,000g離心1分鐘，離心後立即血漿樣本進行殘餘血小板計數檢測。結果顯示，在7,000g離心1分鐘與傳統離心方式，血漿殘餘血小板數無差異，離心效果相同。附加40對樣本測試比較評估以7,000g1分鐘高速離心後是否有產生任何可能的干擾，並影響PT和APTT凝血試驗結果。我們發現兩種離心方式對凝血試驗結果並無統計上的顯著差異。總之，本研究所評估的快速離心處理凝血試驗檢體方法，約可剔除分析前凝血試驗檢體處理時間14分鐘，顯示此高速快速離心是縮短PT和APTT檢驗報告時間(TAT)的一個可靠和有用的方法。
並列摘要
Turnaround time (TAT) is one of the most remarkable signs of laboratory service and is often used as a key performance indicator (KPI) of clinical laboratory. The purpose of this study is to evaluate a new centrifugation method for coagulation testing to provide faster laboratory reports with the aim to improve the TAT thresholds. Six 2mL citrated blood collected from 10 consecutive subjects were centrifuged on a traditional centrifuge at 1500g for 15 minutes and the high-speed centrifuge at 4000g, 5000g, 6000g, 7000g and 8000g for one minute respectively and then supernatant plasma samples were immediately examined for residual platelets. Results revealed consistently achievable equivalence at 7000g for one minute centrifugation in platelet count compared with conventional centrifugation. Additional forty paired samples were tested to determine if any possible interferences after centrifugation at 7000g for one minute affected the results of prothrombin time (PT) and activated partial thromboplastin time (aPTT) tests. We found no significant differences in assay results and conclued that this high-speed technique for preparation of platelet-poor plasma (PPP), shaving about 14 minutes off preanalytical specimen processing time, is a reliable and useful option for minimizing TAT for PT and aPTT tests.
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