毛豆品種（系）及栽培地區對異黃酮之影響

Effect of Cultural Location on the Isoflavone Content and Distribution in Vegetable Soybean Varieties and Its Seed Tissue

李穎宏(Ying-Hung Lee) ； 周國隆(Kuo-Lung Chou) ； 陳正敏(Cheng-Min Chen) ； 龔賢鳳(Hsien-Feng Kung)

《高雄區農業改良場研究彙報》 18卷1期 (2007/06) Pp. 26-52

https://doi.org/10.6958/RBKDAIS.200706.0026

摘要

為發展台灣毛豆產業，本研究以21品系毛豆進行栽培地區（秋作），對異黃酮含量與分佈的影響探討。其中8個芋香毛豆更進一步分離成胚軸與子葉進行異黃酮含量分析。為評估異黃酮分佈變化，12種異黃酮以HPLC進行定量分析，並區分成7個群組分別為TDin、TGlin、TGin、MalGly、Gly、AceGly及AGly。結果顯示：毛豆中異黃酮含量與分佈深受栽培地區與栽培品種的影響。就TDin、TGlin、TGin群組比例大小而言，2栽培地區之異黃酮群組分佈皆具有TGin ＞TDin＞TGlin的型態，但依MalGly，Gly群組比例大小來看，則會因栽培地區的不同而不同。各毛豆品系之異黃酮含量以栽培於萬丹者高於美濃者。栽培於萬丹之毛豆品系除具有較高異黃酮含量外，亦具有較高比例之TGin，及較低比例之TGlin。胚軸具有相當高的異黃酮含量，且glycitein及其另外3種衍生異黃酮，幾乎僅存在此部位內。毛豆中其子葉與全豆的異黃酮分佈相類似，皆為TGin ＞TDin＞TGlin型態，但在胚軸中則以另一型式存在為TDin＞TGlin ＞TGin。栽培於萬丹地區之毛豆，其子葉中所含之異黃酮含量較高，且全豆中之異黃酮含量亦較高，但胚軸中之異黃酮含量則較低。

關鍵字

毛豆 ； 異黃酮 ； 地域 ； 品系

並列摘要

For developing vegetable soybean in Taiwan, the influence of cultural location on the isoflavone contents and distribution in vegetable soybean varieties were investigated. The isoflavone contents and distribution in 21 vegetable soybean varieties sowed in fall were analyzed, and eight of 21 vegetable soybean varieties with taro flavor were further divided into hypocotyls and cotyledons, and the contents and distribution of isoflavone also were analyzed. For evaluating the change of ISF distribution, twelve ISF in whole seeds and seed tissues were quantified by HPLC and sorted into seven ISF groups consist of TDin, TGlin, TGin, MalGly, Gly, AceGly and AGly. Results showed that: Total isoflavone contents of vegetable soybean were significantly affected by cultural location and genotype. For the order of proportion of TDin, TGlin and TGin, vegetable soybean varieties grown at the two location were in a same type of ISF distribution as TGin＞TDin＞TGlin. On the other hand, the order of proportion of MalGly and Gly in vegetable soybean varied at various cultural locations. The isoflavone contents were higher in vegetable soybean grown at Wan-Tan than at Mei-Nung, which also were accompanied with increasing in proportion of TGin and decreasing in proportion of TDin and TGlin. Higher isoflavone contents were concentrated in hypocotyles, and glycitein and its three derivates were almost existed in these seed tissue. The isoflavone distribution in whole bean was similar to that in cotyledon, but different from that in hypocotyle. In whole bean and cotyledon, the propotion of TDin, TGlin, and TGin was in the order of TGin＞TDin＞TGlin. In hypocotyls, that was in a type of TDin＞ TGlin＞ TGin. Accumulation of isoflavones in cotyledon and whole bean were higher for soybean grown at Wan-Tan than at Mei-Nung, but in hypocotyls that was on the opposite tendency.

並列關鍵字

Vegetable soybean ； Isoflavone ； Cultural location ； Hypocotyls