Cytokines and Growth Factors Secreted by Vero Cells Destined for Embryo Cocultures

已知與綠猴腎臟細胞共同培養可以改善胚胎發育。許多研究顯示白血病抑制因子(LIF)，血小板生長因子(PDGF)，變形生長因子(TGF)，和表皮生長因子(EGF)與細胞發育有關。為了定量細胞共同培養所製造之細胞介質又生長因子，綠猴腎臟細胞種入各種培養溶液加上百分之五的小牛血清。我們從第一天到第六天每天收集培養液，沒有加入綠猴腎臟細胞的培養液當做對照組。我們使用的培養液包括HTF，Menezo’s B2，和Medi-cult與綠猴腎臟細胞共同培養時，LIF從第一天就上升，但是隨後濃度就維持穩定；PDGF的濃度從第一天到第六天快速增加；TGFβ1從第一天到第三天逐漸增加，隨後則趨於穩定；EGF則幾乎測不到。相反的，沒有加入綠猴腎臟細胞的對照組所產生的細胞介質及生長因子非常少。我們認為這些細胞介質生長因子在細胞共同培養的助胚胎作扮演一部份的角色。

關鍵字

細胞共同培養；白血病抑制因子；血小板生長因子；變形生長因子；表皮生長因子

並列摘要

It is well established that coculture with Vero cells improves embryo development. Several studies suggest that LIF (leukemia inhibitory fector),PDGF(platelet derived growth factor), TGF(transform-ing growth factor) α and β (now known as TGF β1), and EGF (epidermal growth factor) play an impor-that role in embryo development. In order to quantify the cytokine and growth factors produced by Vero cell coculture, Vero cells were seeded into 6 wells in NUNC 4-well dishes in different culture media plus 5% fetal bovine serum. Culture medium was collected from each well ewvry day for 6 days. Uncon-ditioned medium without Vero cells was used as a control. The culture media used included human tubal fluid (HTF) medium, Menezo’s B2 medium, and Medi-cult universal IVE medium. With Vero cell cocultures, LIF was elevated from day 1 but remained almost constant during the culture interval; PDGF increased rapidly from day 1 through day 6. TGE β1 increased from day 1 to day 3, but plateaued thereafter. EGF, however, was almost undetectable. Unconditioned medium produced little of these cytokine and growth factors. We speculate that these cytokines and growth factors play a role in the embryotrophic action of Vero cell coculture.

並列關鍵字

coculture ； leukemia inhibitory factor ； platelet derived growth factor ； transforming growth factor ； epidermal growth factor

國際替代計量

Cytokines and Growth Factors Secreted by Vero Cells Destined for Embryo Cocultures

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