口腔鱗狀上皮細胞癌中NM23-H1基因產物之免疫組織化學分析

目的：研究口腔鱗狀上皮細胞癌細胞中NM23-H1基因之表現與疾病進展之關聯性。材料與方法：收集大林慈濟綜合醫院2000年至2002年，口腔鱗狀上皮細胞癌病患中，接受腫瘤治療及廣泛切除和頸部淋巴結切除手術，病歷資料和病理切片保存完整的46位病患。（男：女=43：3，年齡：33~88歲、平均54.32歲，頰黏膜22例、舌13例、牙齦11例）。將腫瘤細胞用免疫組織化學分析的方法，研究NM23-H1基因的表現。結果：46位病患整體NM23-H1基因表現是些微增強的（佔54.3%、共25位）。就性別、口腔鱗狀上皮細胞癌部位（頰黏膜、舌或牙齦）、腫瘤分化程度、有無頸部淋巴結轉移、有無遠端轉移，來分析NM23-H1基因表現的強弱，經卡方檢定分析結果並無統計上有意義之區別。結論：本研究歸納出NM23-H1基因在口腔鱗狀上皮細胞癌中有些微較強的表現；但就臨床上而言，口腔鱗狀上皮細胞癌細胞NM23-H1基因的表現有無可行性當作預測腫瘤頸部淋巴結轉移的指標，在本研究中則無法得到進一步的證實。

關鍵字

NM23基因；轉移抑制基因；口腔癌；免疫組織化學分析

並列摘要

Objective: To evaluate the relationship between the MN23-H1 gene product and the disease of oral squamous cell carcinoma. Materials and Methods: Fourty-six subjects who had received tumor ablation surgery and had complete medical records of the oral squamous cell carcinoma (male/female: 43/3; age: 33-88 years old, average, 54.32 years old; buccal mucosa, 22 cases; tongue, 13 cases; and gingival, 11 cases) were selected from 2000 to 2002 in Buddhist Dalin Tzu Chi General Hospital, Taiwan. Expressions of the NM23-H1 gene in these tumor cells were analyzed immunohistochemically. Results: The results show that the expression of the NM23-H1 gene was slightly increased in these 46 cases. There were 25 cases (54.3%) which exhibited positive staining. After analyzing these data with X2 analysis, expression of the NM23-H1 gene was not statistically related to gender (male or female), site (buccal mucosa,　tongue, or gingiva), tumor differentiation (well or non-well), neck lymph nodes metastasis (yes or no), or distant metastasis (yes or no) (p＞0.05). Conclusions: This study shows that expression of the NM23-H1 gene was slightly increased in oral squamous cell carcinoma, but failed to show a clinically, the predictable relationship between neck lymph node metastasis and NM23-H1 gene expression.

並列關鍵字

NM23 gene ； metastasis suppressive gene ； oral cancer ； immunohistochemistry

國際替代計量

口腔鱗狀上皮細胞癌中NM23-H1基因產物之免疫組織化學分析

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