頰黏膜及舌鱗狀上皮細胞癌中NM23-H1基因產物之免疫組織化學分析

Immunohistochemical Evaluation of NM23-H1 Gene Product in Squamous Cell Carcinoma of the Buccal Mucosa and Tongue

黃則達(T.T. Huang) ； 黃穰基(Y.K. Wong) ； 關寶祥(P.C. Kwan) ； 張國威(K.W. Chang) ； 趙守一(S.Y. Chao)

《中華民國口腔顎面外科學會雜誌》 12卷2期 (2001/09) Pp. 81-90

https://doi.org/10.7104/CJOMS.200109.0081

摘要

MN23 gene is identified from murine melanoma cell line in 1998. In its highly metastatic cell line (K1735), the expression of NM23 gene is reduced. The following studys recognized the NM23 gene as a metastatic suppressive gene and identified that reduced expression of NM23-H1 gene is related to highly metastatic potential.
One hundred and six specimens of the buccal mucosa and tongue cancer (squamous cell carcinoma) were selected in Taichuang Veteran General Hospital, Taiwan. The specimens were divided according to genders (male, or female), sites (buccal mucosa, or tongue), tumor differentiated (well, or non-well), neck lymph nodes metastasis (yes, or no) and distant metastasis (yes, or no). The expression of NM23-H1 gene of these specimens were analyzed immunohistochemically. The results show that the expression of NM23-H1 gene is increased in these 106 cases. There are 70 cases (66%) positively staining. Chi square analysis shows that the expression of NM23-H1 gene is not statistically related to the genders (male, of female), sites (buccal mucosa, or tongue), tumor differentiated (well, or non-well), and distant metastasis, there is evidence of slightly reduced expression of NM23-H1 gene (P=0.058). There are 24 cases (42.1%) of negative staining in 57 cases which have neck lymph nodes metastasis compared to 12 cases (24.5%) of negative staining in 49 cases which have no neck lymph nodes metastasis.
This study shows that the expression of NM23-H1 gene is increased in the buccal mucosa and tongue cancer, and in those cases of neck lymph nodes metastasis. There is evidence of slightly reduced expression of Nm23-H1 gene but not statistic significant (P=0.058).

關鍵字

NM23 基因 ； 轉移抑制基因 ； 口腔癌 ； 免疫組織化學分析

並列摘要

MN23 gene is identified from murine melanoma cell line in 1998. In its highly metastatic cell line (K1735), the expression of NM23 gene is reduced. The following studys recognized the NM23 gene as a metastatic suppressive gene and identified that reduced expression of NM23-H1 gene is related to highly metastatic potential.
One hundred and six specimens of the buccal mucosa and tongue cancer (squamous cell carcinoma) were selected in Taichuang Veteran General Hospital, Taiwan. The specimens were divided according to genders (male, or female), sites (buccal mucosa, or tongue), tumor differentiated (well, or non-well), neck lymph nodes metastasis (yes, or no) and distant metastasis (yes, or no). The expression of NM23-H1 gene of these specimens were analyzed immunohistochemically. The results show that the expression of NM23-H1 gene is increased in these 106 cases. There are 70 cases (66%) positively staining. Chi square analysis shows that the expression of NM23-H1 gene is not statistically related to the genders (male, of female), sites (buccal mucosa, or tongue), tumor differentiated (well, or non-well), and distant metastasis, there is evidence of slightly reduced expression of NM23-H1 gene (P=0.058). There are 24 cases (42.1%) of negative staining in 57 cases which have neck lymph nodes metastasis compared to 12 cases (24.5%) of negative staining in 49 cases which have no neck lymph nodes metastasis.
This study shows that the expression of NM23-H1 gene is increased in the buccal mucosa and tongue cancer, and in those cases of neck lymph nodes metastasis. There is evidence of slightly reduced expression of Nm23-H1 gene but not statistic significant (P=0.058).

並列關鍵字

NM 23 gene ； Metastatic suppresive gene ； Oral cancer ； Immunohistochemical evaluation