Quantitative Measurement of Urinary JC Virus Shedding by Real-Time PCR at Different Time Intervals-a Useful Guide for Clinical and Laboratory References

利用Real Time PCR定量量測不同時段的尿液中JCV病毒排放量－臨床及實驗有效的指標

劉素勤(Su-Qin Liu) ； 童建學(Chien-Hsueh Tung) ； 黃光永(Kuang-Yung Huang) ； 林亮宏(Liang-Hung Lin) ； 賴寧生(Ning-Sheng Lai)

《慈濟醫學雜誌》 18卷3期 (2006/06) Pp. 175-178

https://doi.org/10.6440/TZUCMJ.200606.0175

摘要

目的：在人的尿液中可以偵測人類多瘤性病毒(JCV)，但尿液的排放量與物理因素有密切的關係。此實驗我們觀察每位病人每日早、中、晚尿液與病毒量的表現以為臨床及實驗工作的指導。材料與方法：我們先用PCR方法量測140位住院病人JCV病毒，進一步利用定量PCR(quantitative PCR)技術測量病人早、中、晚不同時段的尿液。結果：140位病人接受檢測，其中50位尿液呈現病毒陽性反應。此50位病人早、中、晚尿液皆有JCV病毒。表現量早上清晨第一次排放的病毒量是中午時段病毒排放量的3倍，是晚上的2倍。結論：病毒量的定量表現在臨床診斷及治療上是很主要的參考依據。所以建議，如果要偵測尿液中病毒量的表現，請儘量用病人清晨第一次排放量，使臨床診斷有更好的參考依據與實驗研究有更正確的分析與判讀。

關鍵字

人類多瘤性病毒JCV ； 同步定量real-time PCR ； 病毒量的表現

並列摘要

Objective: Polyomavirus JC virus (JCV) can be detected in human urine and urinary excretion of virus particles may be closely related to physical conditions. For a more sensitive and accurate reference, we quantified virus shedding in urine specimens collected at different time intervals in the morning (first excretion after waking), at noon (between 11 a.m. and 1 p.m.), and in the evening (between 5 p.m. and 6 p.m.). Patients and Methods: Urine samples from 140 hospitalized patients were collected for evaluation of the presence of JCV virus shedding by polymerase chain reaction. Subsequently, urine samples were collected at three different time intervals from those patients with positive urinary shedding on a quantitative real-time PCR. Results: There were 50 patients (36%) who were positive for urinary JCV. Further analysis showed that the median viral load in the first morning urine sample was approximately three times greater than in the noon specimens, and twice as high as in the evening specimens. Conclusions: As quantification of virus shedding is a major reference for clinical viral diagnosis, our findings suggest that monitoring JCV urinary viral load in the first morning urine should provide clinical diagnosis and research a with more sensitive and accurate reference.

並列關鍵字

Polyomavirus JC virus JCV ； quantitative real-time PCR ； viral load