Granulocyte Colony-Stimulating Factor Expression in Bladder Cancer

膀胱癌中白血球生長因子的表現

林漢青(Han-Ching Lin) ； 蔡志仁(Chee-Yin Chai) ； 蘇月秋(Yue-Chiu Su) ； 吳文正(Wen-Jeng Wu) ； 黃俊雄(Chun-Hsiung Huang)

《臺灣泌尿科醫學會雜誌》 18卷2期 (2007/06) Pp. 81-86

摘要

背景：膀胱癌中粒性細胞生長因子的表現常常被提出來討論，有顯著比例的膀胱癌被報導會釋放白血球生長因子。我們收集臨床的資料並且探討其在膀胱癌所扮演的角色。
方法：從1999至2006我們回顧性研究在本院接受根除性膀胱切除且經病理組織切片證實為尿路上皮癌的病人，共有56位病患進入本研究，進行回顧病歷、紀錄疾病分期並追蹤病患存活的資料外，也調出當時術後檢體，用特定的G-CSF抗體進行免疫組織染色法以確認該組織G-CSF的表現。
結果：56位病患接受根除性膀胱切除，其術後檢體進行免疫組織染色法，所有非腫瘤的部份對G-CSF的表現皆呈陰性反應。我們將腫瘤染色的結果分為兩組（高度反應及低度反應），並且發現其與病人術後存活有顯著相關(p=0.008)。而且似乎跟腫瘤的分期無明顯的相關，而組織G-CSF的表現和術前的白血球增生和泌尿道感染有顯著的相關(p=0.009 and 0.04, respectively)。
結論：組織G-CSF的表現發現其與病人術後存活有顯著相關，我們無法用其與腫瘤的侵犯程度作解釋。但卻發現組織G-CSF的表現和術前的白血球增生和泌尿道感染有顯著的相關。嚴格的控制術前的感染對病人的術後存活率是有幫助的。

關鍵字

白血球生長因子 ； 膀胱癌 ； 組織晶片 ； 存活率

並列摘要

OBJECTIVE: Much research has discussed the expression of granulocyte colony-stimulating factor (G-CSF) in urothelial cancer but few clinical data are available. A significant proportion of bladder carcinomas have been reported to express G-CSF. Our study examined the role of G-CSF expression in bladder cancer.
MATERIALS AND METHODS: From 1999 to 2006, we collected data on 56 cases who received a radical cystectomy at Kaohsiung Medical University Hospital. Clinical data including TNM stage, grade, laboratory data, and infective status were reviewed. Expressions of G-CSF in the tumors were detected by immunohistochemical staining. We examined the association of the expression of G-CSF with the tumor TNM stage, grade, and infectious status by Chi-squared or Fisher's exact test.
RESULTS: The immunohistochemical staining for G-CSF was performed on tissues from 56 patients with bladder cancer after a radical cystectomy. All of the non-tumor parts of the urinary bladder negatively stained for G-CSF. We divided the results of staining into 2 parts: low and high. We found there was a negative association between the G-CSF expression intensity and patient postoperative survival (p=0.008). However, no significant relationships between G-CSF expression and tumor TNM stage or grade were identified. We did, however, find positive associations between G-CSF levels and preoperative leukocytosis and urinary tract infection (p=0.009 and 0.04, respectively).
CONCLUSIONS: In the present study, G-CSF expression in bladder cancer was proven to be negatively associated with postoperative survival in patients who received a radical cystectomy. This cannot be explained by the invasiveness of the tumor when we compared the expression of G-CSF with the tumor TNM stage and grade. We also found that preoperative leukocytosis and urinary tract infection were positively associated with the intensity of G-CSF expression. Strict control of these problems may be beneficial to patients who are to receive a radical cystectomy.

並列關鍵字

granulocyte colony-stimulating factor ； bladder cancer ； tissue array ； survival