兩側足三里穴注射黃耆、當歸混合液對甲基硝基亞硝基胍誘發大鼠胃粘膜組織損傷及胃粘液屏障的影響

The Effect of Huang-Qi and Dong-Kwa Mixture Solution on N-Methyl-N-Nitro-Nmitroso-Quanidine Induced Damage of Gastric Mucous Membrane and Gastric Mucous Barrier in SD Rats: A Study Using Bilateral Zusanli Acupoints (ST-36)

孫茂峰(Mao-Feng Sun) ； 王茵萍(Yin-Ping Wang) ； 林昭庚(Jaung-Geng Lin) ； 謝慶良(Ching-Liang Hsieh)

《中醫藥雜誌》 12卷4期 (2001/12) Pp. 251-269

https://doi.org/10.6940/JCM.200112_12(4).04

摘要

兩側足三里穴注射黃耆和當歸混合液來治療慢性萎縮性胃炎（chronic atrophic gastritis，以下簡稱CAG）在臨床上已被認為有效，而一般認為慢性萎縮性胃炎的發生與胃粘膜組織及胃粘液屏障的損傷有密切的關係。因此，本研究更進一步探討了兩側足三里穴注射黃耆和當歸混合液對胃粘膜組織及胃壁屏障的影響。方法是將分別自由飲用甲基硝基亞硝基胍(N-methyl-Nnitro-N-mitroso-quanidine, MNNG)溶液40μg/ml和60μg/ml的Sprague-Dawley(SD)大鼠，經九周和三十一周後將它們部份犧牲觀察胃粘膜損傷、萎縮、腸化(metaplasia)和異常增生的程度，並於第十周到三十一周間施行注射黃耆和當歸混合液來與對照組做比較。根據胃粘膜損傷、萎縮、腸化生和異常增生的程度，以及氨基己糖和磷脂濃度的變化當做指標來做為穴位注射的療效評估。結果發現胃粘膜損傷、萎縮、腸化生和異常增生的程度與服用MNNG溶液的濃度和時間呈現正的相關關係，同時服用甲基硝基亞硝基胍溶液也能使氨基己糖和磷脂濃度下降，但於兩側足三里穴施行注射黃耆和當歸混合液的大鼠，這些現象較為輕微，同時它們的氨基己糖和磷脂濃度也較高。
我們的結論是兩側足三里穴施行注射黃耆和當歸混合液可以減輕甲基硝基亞硝基胍溶液所誘發的胃粘膜損傷、萎縮、腸化和異常增生，以及氨基己糖和磷脂的減少，並且於兩側足三里穴施行注射黃耆和當歸混合液可以保護胃粘膜組織的損傷和胃粘液屏障，因此可用來治療人類胃部有關粘膜損傷的疾病，如慢性萎縮性胃炎等。

關鍵字

黃耆當歸混合液 ； 胃粘膜 ； 胃粘膜屏障 ； 足三里穴 ； 甲基硝基亞硝基胍

並列摘要

The injection of Huang-Qi (黃耆) and Dong-Kwa (當歸) mixture solution at bilateral Zusanli acupoints (ST-36) has been used to treat chronic atrophic gastritis (CAG). Several studies found that the generation of CAG has closely related to damage of gastric mucous membrane and gastric mucous barrier, therefore, the aim of the present study was to investigate the effect of Huang-Qi and Dong-Kwa mixture solution that was injected into bilateral Zusanli acupoints on gastric mucous membrane and gastric mucous barrier. The animal model in the Sprague-Dawley (SD) rats was established by oral administration of Nmethyl- N-nitro-N-mitroso-quanidine (MNNG) 40 μg/ml or 60 μg/ml, respectively. The rats were sacrificed and pathological changes of gastric mucous membrane were observed including erosion, atrophy, metaplasia and hyperplasia 9 weeks or 31 weeks after MNNG administration. In addition, Huang- Qi and Dong-Kwa mixture solution was injected into bilateral Zusanli acupoints since 10th to 31th week. Effect of Huang-Qi and Dong-Kwa mixture solution was evaluated by changes of erosion, atrophy, metaplasia and hyperplasia in the gastric mucous membrane, and the levels of hexosamine and phospholipid. The results indicated that gastric mucous membrane developed to erosion, atrophy, metaplasia and hyperplasia, and the levels of both hexosamine and phospholipid decreased after administration of MNNG. The degree of these changes had a positive correlation to the concentration and duration of MNNG administration. In addition, these changes were milder in the group of MNNG administration than in the control group without MNNG administration.
In conclusion, Huang-Qi and Dong-Kwa mixture solution injected into the bilateral Zusanli acupoints may decrease the damage of gastric mucous membrane including erosion, atrophy, metaplasia and hyperplasia, and may prevent the decrease of hexosamine and phopholipid levels in the MNNGtreated rats, suggesting Huang-Qi and Dong-Kwa mixture solution injected into the bilateral Zusanli acupoints may produce a protective action to gastric mucous membrane and gastric mucous barrier, therefore, it may be used to treat gastric diseases that result from the damage of gastric mucous membrane, such as CAG.

並列關鍵字

Huang-Qi and Dong-Kwa Solution ； Gastric Mucosa ； Gastric Mucous Barrier ； Zusanli Acupoints ； N-Methyl-N-Nitro-N-Mitroso-Quanidine MNNG