台灣與中國大陸產吳茱萸及其同屬植物之活性成分Dehydroevodiamine, Evodiamine, Rutaecarpine及Synephrine的含量比較

Determination of Dehydroevodiamine, Evodiamine, Rutaecarpine and Synephrine in Evodia Genus Plants from Taiwan and Mainland China

柯漢傑(Han-Chieh Ko) ； 陳國棟(Kuo-Tung Chen) ； 周正仁(Cheng-Jen Chou) ； 陳介甫(Chieh-Fu Chen)

《中醫藥雜誌》 13卷3期 (2002/09) Pp. 151-158

https://doi.org/10.6940/JCM.200209_13(3).04

摘要

中藥吳茱萸為芸香科(Rutaceae)植物吳茱萸Evodia rutaecarpa (Juss.) Benth之未成熟乾燥果實，為我國傳統常用藥物之一。神農本草經將吳茱萸列為草部中品，可治胃痛、頭痛、腹痛、痢疾、產後出血及停經等疾病，現代藥理學研究發現其活性成分dehydroevodiamine(Ⅰ)，evodiamine(Ⅱ)，rutaecarpine(Ⅲ)有降血壓、抗心律不整、刺激內皮細胞及抑制巨噬細胞釋放一氧化氮之作用，而synephrine(Ⅳ)具有升血壓之作用。本研究之目的在利用高效液相層析法，對台灣與中國大陸產吳茱萸及台灣產同屬植物賊仔樹Tetradium glabrifolium (Champ. Ex Benth.) T.Hartley果實中上述四種活性成分含量之異同研究。
本實驗是使用HP-1100高效液相層析儀系統：分離管柱為C18(Reversed-phaseC18 column)，移動相選擇acetonitrile, water, sodium dodecyl sulfate, orthophosphoric acid solution(85%)的混合緩衝液，以線性梯度程式沖提，流速為1ml/min,以UV270nm波長偵測。
經上述方法分析結果，四種成分在中國大陸產吳茱萸中之含量分別：Ⅰ為3.00±0.06mg/g，Ⅱ為3.39±0.18mg/g，Ⅲ為4.02±0.30mg/g；台灣產吳茱萸中之含量：Ⅰ為1.02±0.06mg/g，Ⅱ為0.40±0.01mg/g, Ⅲ為0.69±0.03mg/g；同屬植物賊仔樹果實中之含量為：Ⅰ為0.76±0.13mg/g, Ⅱ為1.79±0.37mg/g, Ⅲ為0.92±0.19mg/g，而Ⅳ為0.62±0.06mg/g僅在中國大陸產吳茱萸中發現。

關鍵字

吳茱萸 ； 臭辣樹 ； 賊仔樹 ； 高效液相層析 ； 含量分析

並列摘要

Wu-Chu-Yu, the unriped fruit of Evodia rutaecarpa (Juss.) Benth., is a commonly used Chinese herbal drug, which has been classified as ”middle class” in Shen-Nong-Ben-Cao-Jing and for the treatment of gastrointestinal disorders, headache, abdominal pain, dysentery, postpartum hemorrhage and amenorrhea. We found that three alkaloids, dehydroevodiamine (Ⅰ), evodiamine (Ⅱ), and rutaecarpine (Ⅲ), obtained from Wu-Chu-Yu, possess hypotensive and antiarrhythmic effects, stimulated endothelial cells and inhibit ed macrophages nitric oxide release. While, another bioactive compound, synephrine (Ⅳ), possess hypertensive effect.
In this study, a high performance liquid chromatographic method was developed for simultaneous determination of these four components in Evodia rutaecarpa and Evodia meliaefolia obtained from mainland China and Taiwan.
The analysis was made by using a gradient elution with a mobile phase solution of acetonitrile, water, sodium dodecyl sulfonate (SDS), orthophosphoric acid (85%) on a C18 column (Reversedphase C18 column), the flow rate was 1.0 ml/min, and detect ed by UV (270 nm).
The results demonstrated that 3.00±0.06 mg/g of Ⅰ, 3.39±0.18 mg/g of Ⅱ, 4.02±0.30 mg/g of Ⅲ were contained in Evodia rutaecarpa obtained from mainland China; 1.02±0.06 mg/g of Ⅰ, 0.40±0.01 mg/g of Ⅱ, 0.69±0.03 mg/g of Ⅲ and 0.76±0.13 mg/g of Ⅰ, 1.79±0.37 mg/g of Ⅱ, 0.92±0.19 mg/g of Ⅲ were contained in Evodia rutaecarpa obtained from Taiwan. and Evodia meliaefolia obtained from Taiwan respectively. While, synephrine (0.62±0.06 mg/g) was only detected in Evodia rutaecarpa obtained from mainland China.

並列關鍵字

Evodia ； Tetradium ； Dehydroevodiamine ； Evodiamine ； Rutaecarpine ； Synephrine ； Highperformance liquid chromatography