蛇床子萃取物於抗氧化、抑制黑色素生成及抗致突變的研究

Study of Cnidium Monnieri Fruits Extract on the Effects of Antioxidation, Inhibiting the Formation of Melanin and Antimutagenicity

曾文楷(Wen-Kai Tseng) ； 陳宜嫻(Yi-Shyan Chen) ； 官常慶(Chang-Chin Kwan)

《中醫藥雜誌》 19卷3&4期 (2008/12) Pp. 123-133

https://doi.org/10.6940/JCM.200812_19(3_4).05

摘要

本研究採用蛇床子(Cnidium monnieri (L.) Cuss.)的乾燥成熟果實為材料，經95%乙醇萃取後，所製得的蛇床子乙醇萃取物再進行抗氧化性、抑制黑色素生成的效果以及抗致突變性等試驗。在抗氧化方面，100.0 mg/ml蛇床子萃取物清除DPPH自由基的效果相當於同濃度Ascorbic acid的68.4%、BHA的67.9%以及BHT的67.6%；100.0 mg/ml蛇床子萃取物的還原力相當於同濃度Ascorbic acid的75.6%、BHA的80.3%以及BHT的83.6%；25.0 mg/ml蛇床子萃取物螯合亞鐵離子的效果相當於同濃度EDTA的93.3%。在抑制黑色素生成方面，蛇床子萃取物抑制生成黑色素的中間產物Dopachrome的IC50為68.70 mg/ml。另由沙門氏菌回復突變性試驗之結果顯示蛇床子萃取物不具致突變性，而且0.1 mg/plate蛇床子萃取物對4-NQO引起致Salmonella typhimurium TA98突變的抑制率為26.9%，0.05 mg/plate蛇床子萃取物對4-NQO引起致Salmonella typhimurium TA100突變的抑制率為38.6%，均表示有輕度活性的抗致突變作用。綜合上述結果，蛇床子萃取物為一安全而具有化學防護效果的中草藥，應可再進一步進行活性成分的分析研究，以確定其於抗老化、抑制黑色素沉著方面的應用價值。

關鍵字

蛇床子 ； 抗氧化 ； 黑色素 ； 抗致突變

並列摘要

The dried fruits of Cnidium monnieri were extracted by 95% ethanol, the ethanolic extract was examined on the effects in the antioxidative activity, the inhibiting the formation of dopachrome and the antimutagenicity. In the concentration of 100.0 mg/ml, the ability of the extract for the scavenging DPPH free radicals is 68.4%, 67.9% and 67.6%, while, for the reducing ferric ion is 75.6%, 80.3% and 83.6% to ascorbic acid, BHA, and BHT, respectively. In 25.0 mg/ml, the ability for the chelating ferrous ion is 93.3% to EDTA. The above results indicate the extract behaves antioxidative activity. The IC50 of the extract for the formation of dopachrome is 68.70 mg/ml. Indicating that the extract shows the activity for inhibiting melanogenesis. The extract does not possess mutagenicity. The suppressing rate of the extract with 0.1 mg/plate for the mutation of Salmonella typhimurium TA98 caused by 4-NQO is 26.9%, while the suppressing rate of the extract with 0.05 mg/plate for the mutation of Salmonella typhimurium TA100 caused by 4-NQO is 38.6%. Suggesting that the extract belongs to the weak activity group of antimutagenicity. In conclusion, the extract of Cnidium monnieri fruits is a kind of safe chemopreventer and should be analyzed its active components further. These components may be used for preventing aging and inhibiting pigmentation.

並列關鍵字

Cnidium monnieri fruits ； antioxidation ； melanin ； antimutagenicity