建立骨質疏鬆症之動物模式－初報

Development of Osteoporosis Animal Models-Preliminary Report

陳慰宗(Wei-Tsung Chen) ； 施庭芳(Tiffany Ting-Fang Shih) ； 李碧珍(Pi-Jen Lee) ； 李亞倫(Yan-Luen Lin) ； 陳潤秋(Ran-Chou Chen) ； 杜興洋(Shin-Yang Tu) ； 璩大成(Da-Chen Chu)

《中華放射線醫學雜誌》 29卷2期 (2004/04) Pp. 67-72

摘要

Background: The purpose of this study was to develop osteoporosis animal models by control a single risk factor for different kinds of osteoporosis, such as :to simulate postmenopausal osteoporosis by ovariectomy, to simulate steroid-induced osteoporosis by daily methylprednisolone injection, to simulate immobilization osteoporosis by fixation of unilateral hind leg.
Materials and methods: We performed: (1) ovariectomy on 9 female Wistar rats, (2) daily injection of methylprednisolone sodium succinate (1ug/g/dau) on 5 male Wistar rats, (3) fixation of unilateral hind leg on 7 male Wistar rats. Quantitative computer tomography in association with quantitative phantom was used to calculate the bone mineral densities of trabecular bone, cortex and the combined values in each subject. Statistical analyses were performed to compare the bone mineral densities between treatment and control groups.
Results: Significant bone mineral density decrease (p＜0.05) developed in cortical bone, trabecular bone and combined measurement of both structures in treatment groups of the 3 models, as compared to their control groups. In ovariectomy and steroid-injection animal models, the extent of bone loss in trabecular bones was more prominent that in cortical bones (28.2% versus 4% and 28.4% versus 10%). In disuse animal model, the extent of bone loss in trabecular and cortical bones was similar (10% versus 6.2%).
Conclusion: Our results demonstrate successful induction of bone loss by different treatments, which can be used as proper osteoporosis animal models for basic studies and evaluation of effectiveness of new drug for osteoporosis in the future.

關鍵字

動物模式 ； 骨質疏鬆症

並列摘要

Background: The purpose of this study was to develop osteoporosis animal models by control a single risk factor for different kinds of osteoporosis, such as :to simulate postmenopausal osteoporosis by ovariectomy, to simulate steroid-induced osteoporosis by daily methylprednisolone injection, to simulate immobilization osteoporosis by fixation of unilateral hind leg.
Materials and methods: We performed: (1) ovariectomy on 9 female Wistar rats, (2) daily injection of methylprednisolone sodium succinate (1ug/g/dau) on 5 male Wistar rats, (3) fixation of unilateral hind leg on 7 male Wistar rats. Quantitative computer tomography in association with quantitative phantom was used to calculate the bone mineral densities of trabecular bone, cortex and the combined values in each subject. Statistical analyses were performed to compare the bone mineral densities between treatment and control groups.
Results: Significant bone mineral density decrease (p＜0.05) developed in cortical bone, trabecular bone and combined measurement of both structures in treatment groups of the 3 models, as compared to their control groups. In ovariectomy and steroid-injection animal models, the extent of bone loss in trabecular bones was more prominent that in cortical bones (28.2% versus 4% and 28.4% versus 10%). In disuse animal model, the extent of bone loss in trabecular and cortical bones was similar (10% versus 6.2%).
Conclusion: Our results demonstrate successful induction of bone loss by different treatments, which can be used as proper osteoporosis animal models for basic studies and evaluation of effectiveness of new drug for osteoporosis in the future.

並列關鍵字

Animal mode ； Osteoporosis