A colorimetric method was used to study the residence time distribution (RTD) in an APV Baker MPF 50/25 twin-screw extruder using rice flour as the feed material. The process variables were screw profile, feed rate, and screw speed. A 3×3×3 factorial experiment was designed to study the effects of process variables on residence time distributions. The effects of process variables on the mean residence time were significant. In general, increasing the number of forward paddles in the extruder metering zone, feed rate, or screw speed decreased the mean residence time and shifted the RTD E curve to the left. However, the influences of process variables on the RTD spread were less significant than on the mean residence time.