摘要
軌道阱質譜術為近年來所發展的新型高解析質譜技術，其原理為利用高靜電場力方式將離子捕捉於特殊的軌道阱中，離子在此阱中運動時將產生特殊的圓周半徑以及震盪頻率，而此頻率與質荷比有關，經由傅立葉轉換方式可以將其轉變為該離子之精確質量數。軌道阱質譜術具有低背景訊號、高靈敏度、高質量準確度、寬廣的動態範圍以及操作簡單等優點，目前已廣泛地應用於生物分析領域。本文將介紹其原理以及儀器構造，並介紹該技術於蛋白質體學、小分子結構鑑定、藥物以及環境分析方面的應用。
關鍵字
無資料並列摘要
Orbitrap mass spectrometry is a new mass technique for high resolution mass spectrometry discovered recently. The gas ion will have a special radius and frequency via high electrostatic force when it moves in the orbitrap. The frequency in Z axis has a mathematical relationship with m/z. The accurate mass of each ion can be measured after the frequency is transformed with Fourier transform equation. Due to the advantages of low background noise, high sensitivity, high mass accuracy, wide dynamic range and easy use, the orbitrap mass spectrometry has been largely applied in biological analysis. This paper will introduce the theory and instrumental structure of orbitrap mass spectrometer. The applications of orbitrap mass spectrometry in the proteomics, chemical structure identification of small molecules, drug and environmental analysis are also reviewed.