Effects of Epidermal Growth Factor on the Growth of Human Uveal Melanoma Cells and Uveal Melanocytes in Vitro

表皮生長因子在體外對人類葡萄膜黑色素瘤細胞及正常葡萄膜黑色素細胞生長之作用

林靖洋(Ching-Yang Lin) ； 林浤裕(Hung-Yu Lin) ； 胡誕寧(Dan-Ning Hu) ； Steven A. McCormick

《中華民國眼科醫學會雜誌》 46卷4期 (2007/12) Pp. 408-414

https://doi.org/10.30048/ACTASOS.200712.0003

摘要

目的：之前已有報告人類葡萄膜黑色素瘤細胞有表皮生長因子受體(EGFR)存在，但尚未有根據測試葡萄膜黑色素瘤細胞對表皮生長因子(EGF)之反應以證實此EGFR具有功能。本文係測試EGF在體外對人類葡萄膜黑色素瘤細胞及葡萄膜黑色素細胞生長之作用，以驗證葡萄膜黑色素瘤細胞之EGFR是否具有功能。
方法：從原發的脈絡膜黑色素瘤標本分離並培養葡萄膜黑色素瘤細胞；從捐獻之屍體眼球分離並培養正常葡萄膜黑色素細胞。將葡萄膜黑色素瘤細胞與葡萄膜黑色素細胞分別接種至多皿板。加入不同濃度之EGF進行培養，每組三皿。以不加EGF之培養作為對照組。培養6天後將細胞脫下，計數並進行比較。
結果：EGF能明顯促進葡萄膜黑色素瘤細胞之生長，且在濃度1-100 ng/ml之範圍內呈現劑量效應。用100 ng/ml EGF培養之葡萄膜黑色素瘤細胞計數為對照組之151±13%。EGF(1-100 ng/ml)對葡萄膜黑色素細胞之生長無明顯作用。
結論：EGF刺激葡萄膜黑色素瘤細胞之生長但不影響正常葡萄膜黑色素細胞之生長，證實葡萄膜黑色素瘤細胞有功能性EGFR之存在，此可能為導致葡萄膜黑色素瘤細胞無限制地生長的原因之一。

並列摘要

Purpose: It has been reported that epidermal growth factor receptors (EGFR) present in some uveal melanoma specimens and cell lines. However, the validation of these EGFR is functional by testing the response of melanoma cells to exogenous EGF has not been reported. The purposes of this study was to study the effects of epithelial growth factor (EGF) on the growth of cultured human uveal melanocytes and uveal melanoma cells to document the presence of functional EGFR in uveal melanoma cells.
Methods: Immortal human choroidal melanoma cell line (TP31) from surgical specimen from primary choroidal melanoma was used. Human uveal melanocytes were isolated and cultured from donor eyes. Early passages of three cell lines of uveal melanocytes and this cell uveal melanoma cell line were plated separately into multi-well plates and cultured. EGF was added to the culture medium at various concentrations. Each group was performed in triplicate. Cells cultured with the culture medium without EGF were used as the controls. After 6 days, cells were detached and counted.
Results: EGF significantly stimulated the growth of uveal melanoma cells in a dose dependent manner from 1-100 ng/ml. The cell number of EGF treated groups (100 ng/ml) was 151±11% of the controls (p＜0.01). EGF did not have any significant effect on the growth of uveal melanocytes at the concentration from 1-100 ng/ml (p＞0.05).
Conclusion: EGF stimulates the growth of uveal melanoma cells but not normal uveal melanocytes. This indicates that functional EGFR present in uveal melanoma cells. This may be one of the factors that lead to unlimited growth of uveal melanoma cells in vivo.

並列關鍵字

EGF ； uveal melanoma ； uveal melanocyte ； growth ； EGFR