Parthenogenetic Reproduction by the Entomopathogenic Nematode, Steinernema carpocapsae (Nematoda: Steinernematidae), on Poultry Egg Yolk or Galleria Larva

蟲生線蟲Steinernema carpocapsae在家禽蛋黃或臘蛾幼蟲培養基上之孤雌生殖

蕭文鳳(Wen-Feng Hsiao) ； 朱亮光(Liang-Kuang Chu)

《Zoological Studies》 35卷3期 (1996/07) Pp. 227-229

摘要

本研究係在三個不同的實驗室內進行以觀察蟲生線蟲Steinernema carpocapsae不同品系的生殖方式。在所製備的直徑9公分之石膏培養皿中央挖直徑1公分的洞，並分別填滿經熱處理過的末齡蠟蛾幼蟲及等量殺過菌的蛋黃。爾後每皿再接入一隻致病性線蟲幼蟲，測試品系有Strains 25，DD-136，和AII品系三種。並以不接種致病性線蟲幼蟲為對照，接種7天後，每日觀察其發育情形並計數所產後代數。每皿視為一重複，每品系每種培養基重複20次。結果顯示其中以卵黃為培養基者，產生後代的比率，Strains 25及DD-136皆為45%，AII品系為55%，所產後代數Strain 25相當低，都在100隻範圍內，而DD-136品系，後代數分布在10^3－10^4之間，以10^3比率最高。AII品系分布的等級分布在10^2－10^4之間，以10^3比率最高。以蠟蛾為培養基者，產生後代的比率，Strains 25及AII皆為50%，DD-136品系為55%，所產後代數Strain 25和蛋黃培養基所飼養者相同皆相當低，在100隻範圍內，而DD-136品系，後代數分布在10^3－10^4之間，以10^3比率最高。AII品系分布的等級分布在10^2－10^4之間，以10^3比率最高。實驗結果顯示蟲生線蟲Steinernema carpocapsae在特殊情況下亦能行孤雌生殖。

關鍵字

孤雌生殖 ； 子代 ； 家禽蛋黃

並列摘要

Three strains of Steinernema carpocapsae (strains 25, DD-136, and AII) were used to investigate the possibility of parthenogenesis in this species of entomopathoganic nematode. A plaster plate with a depression (dia. of 1 cm) in the center was designed for this experiment. Heat-treated last instar Galleria larvae or an equivalent weight of autoclaved poultry egg yolk were used in separate experiment to fill up the hole. A single S. carpocapsae infective juvenile was inoculated onto each sample of medium. Each plaster plate was assigned as 1 replicate and each treatment consisted of 20 replicates. For both media plates without nematode inoculation were assigned as the control. Development was observed daily and the number of progeny produced per nematode was counted 7 days after inoculation. For the yolk medium, the ratios of replicates producing progeny were 45% for strains 25 and DD-136, and 55% for strain AII. The number of progeny produced by strain 25 was below 100. The number of progeny produced by DD-136 ranged from 10^3 to 10^4 with a high proportion at 10^3. For strain AII, the number of progeny produced ranged from 10^2 to 10^4 with a high proportion at 10^3. For the Galleria larva medium, the ratios of replicates producing progeny were 50% for strains 25 and AII, and 55% for strain DD-136. The number of progeny produced by strain 25 was below 100. The number of progeny produced by DD-136 ranged from 10^3 to 10^4 with a high proportion at 10^3. For strain AII, the number of progeny produced ranged from 10^2 to 10^4 with a high proportion at 10^3.

並列關鍵字

Parthenogenesis ； Steinernema carpocapsae ； Progeny ； Poultry egg yolk ； Galleria