Growth Promotion in Ayu (Plecoglossus altivelis) by Gene Transfer of the Rainbow Trout Growth Hormone Gene

我們利用精子電穿孔轉殖法將含鯉魚β-肌動蛋白啟動子的虹鱒生長激素cDNA質體轉殖到香魚受精卵中。基因轉殖香魚之孵化率及存活率都較控制組低，且在十萬個處理過的受精卵中約有10%的育成率。經過5個月飼養後，萃取尾鰭基因體DNA，利用特定引子進行聚合酶鏈鎖反應來檢測轉殖基因。聚合酶鏈鎖反應和限制酶切割分析證實基因轉殖成功率約為55%。以反轉錄酶－聚合酶鏈鎖反應來分析轉殖基因之表現，發現其表現型為鑲嵌式，可在鰓、尾鰭、肝臟、腦和肌肉中表現，而不在心臟表現。此基因轉殖香魚促進生長之效率，以體重而言為非基因轉殖組的2倍，體長則為非基因轉殖組的1.3倍。

關鍵字

基因轉殖；香魚；精子電穿孔法；生長激素

並列摘要

A plasmid containing rainbow trout growth hormone cDNA under a carp β-actin promoter was transferred into ayu (Plecoglossus altivelis) using a sperm-electroporation method. Hatching and survival rates of ayu into which the gene was transferred were lower than those of the control, and about 10% of ayu were raised from 105 treated fertilized eggs. The persistence of transferred DNA was detected using polymerase chain reaction (PCR) with specific primers in genomic DNA from caudal fin tissue after 5 mo. PCR and restriction enzyme digestion analyses showed that the success of gene transfer was as high as 55%. Mosaic expressions of the transgene could be detected in gill, caudal fin, liver, brain, and muscle, but not in the heart. Two-fold growth enhancement in body weight and 1.3-fold in body length were measured.

並列關鍵字

Gene transfer ； Ayu ； Sperm-electroporation ； Growth hormone

國際替代計量

Growth Promotion in Ayu (Plecoglossus altivelis) by Gene Transfer of the Rainbow Trout Growth Hormone Gene

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