- 期刊
Inheritance of Microsatellite Loci and Their Application for Pedigree Analysis of the Polyploid Persian Sturgeon Acipenser Persicus (Acipenseridae)
並列摘要
Identification of suitable molecular markers for correctly assigning progenies to their parents particularly during the early stages of development is vital for aquaculture breeding programs. Developing such markers in sturgeons, that have a polyploid ancestry, is particularly challenging because many markers exhibit polysomic inheritance. In the present study, 2 F1 families of 23 and 28 larvae were produced in 1 × 1 crosses of Persian sturgeon. Eleven microsatellite loci were used to genotype the parents and their offspring. An analysis of inheritance patterns demonstrated classical mendelian disomic inheritance in all but 2 of the markers tested in the offspring. Two loci exhibited inheritance patterns consistent with a parental null allele that was consistently inherited by approximately 1/2 of the offspring screened. Thus, the markers tested here can be used for parental assignment testing and for population genetic studies. They can also be used as reliable molecular markers for constructing a genetic linkage map for the target species that will be essential for future quantitative trait locus mapping purposes. This is the 1st report of mendelian segregation testing in Persian sturgeon for cross-species amplification of single-locus DNA microsatellite markers.
延伸閱讀
- Ku, F. C., Yao, C. J., Lin, R. C., Chou, L. S., & Li, S. H. (2020). Isolation and Characterization of Seven Tetrarepeat Microsatellite Loci in the Indo-Pacific Finless Porpoise (Neophocaena phocaenoides) and Their Amplification across Cetacean Species. Collection and Research, (33), 1-7. https://doi.org/10.6693/CAR.202012_(33).0001
- Ji, Y., Zhang, G. Z., & Zhang, Y. H. (2016). Optimization of Enzymatic Hydrolysis of Protein from Small Yellow Croaker (Psendosciaena polyactis) Using Response Surface Methodology. Advance Journal of Food Science and Technology, 11(1), 1-6. https://doi.org/10.19026/ajfst.11.2346
- Liu, A. C. H. (2018). Evaluation of extraction and analysis protocols for polystyrene microplastics ingested by copepods using Pseudodiaptomus annandalei [master's thesis, National Taiwan University]. Airiti Library. https://doi.org/10.6342/NTU201801282
- Casares, M. V., Cabo, L. I. D., Seoane, R. S., Natale, O. E., Ríos, M. C., Weigandt, C., & Iorio, A. F. D. (2012). Measured Copper Toxicity to Cnesterodon decemmaculatus (Pisces: Poeciliidae) and Predicted by Biotic Ligand Model in Pilcomayo River Water: A Step for a Cross-Fish-Species Extrapolation. Journal of Toxicology, 2012(), 608-618. https://doi.org/10.1155/2012/852384
- Yang, W. C., Pang, V. F., Jeng, C. R., & Chueh, L. L. (2012). Short communication: Localization of Morbillivirus Nucleic Acid in a Pygmy Sperm Whale (Kogia breviceps) by In Situ-Reverse Transcriptase-Polymerase Chain Reaction. 臺灣獸醫學雜誌, 38(3), 177-182. https://doi.org/10.7009/TVJ.201209.0177