The protection forest is distributed scattering among the island of Taiwan, its function in safeguarding the territory is very important. How to establish an efficient protection forest management policy is a main task for the manager. This study focused on how to use a new technique of remote sensing to research landscape structure of the protection forest within Kenting National Park. Multivariate analysis was used to group the 20 protection forest into 3 groups, and according to the landscape ecology theory to name them as diversity landscape structure, simple landscape structure and fragmentation landscape structure. Moreover we select NP (number of patches), ED (edge density) and SHDI (Shannon's diversity indices) index to assess the disturbance of protection forest. The result showed that the region which protection forest impact seriously was that the human activity frequently or northeast monsoon attacked area.