A Novel Method for the Determination of Pectinesterase Inhibitor in Banana

香蕉果膠酯酶抑制劑新測定方法之研究

王裕泰(Yuh-Tai Wang) ； 吳柏志(Bo-Jhih Wu) ； 張鴻民(Hung-Min Chang) ； 吳瑞碧(James Swi-Bea Wu)

《藥物食品分析》 15卷2期 (2007/06) Pp. 185-190

摘要

本文研究一個利用酵素免疫分析法(ELISA)來測定香蕉果膠酯酶抑制劑(PEI)的新方法。這個方法是利用PEI和PE之間以及PE、兔子anti-PE多株抗體和鹼性磷酸酶結合的羊抗兔免疫球蛋白之間的選擇性鍵結來進行分析。研究發現：ELISA方法比常用的pH-stat方法更能敏感地定量香蕉、愛玉子瘦果和奇異果中的PEI。因此，本方法提供一個保存原料和有助於分析新鮮和加工果汁中PEI的好選擇。此外，經由ELISA方法和pH-stat方法皆證實香蕉在低溫貯存的熟成遲延與PEI活性有關。

關鍵字

果膠酯酶 ； 抑制劑 ； pH-stat方法 ； ELISA ； 後熟

並列摘要

A novel enzyme-linked immunosorbent assay (ELISA) method for the quantification of pectinesterase inhibitor (PEI) in banana was described. The method was based on the specific binding of PE with PEI, rabbit anti-PE polyclonal antibody and goat antirabbit IgG-conjugated alkaline phosphatase in series. The ELISA method was found to be more sensitive to quantify PEI in banana, jelly fig (Ficus awkeotsang Makino) achene, and kiwi than the conventional pH-stat method. It thus provides a good alternative that saves raw material and efforts in the analysis of PEI in both fresh and processed fruits. The retardation in the ripening of banana pulp in low temperature storage was proven relevant to the maintenance of PEI activity as determined by both methods.

並列關鍵字

pectinesterase ； inhibitor ； pH-stat method ； ELISA ； postharvest