Direct Solid-phase Spectrophotometric Determination of Tartrazine in Soft Drinks Using β-Cyclodextrin Polymer as Support

以β-環糊精聚合物作爲支持體直接光度法測定清涼飲料中的檸檬黃

李榮(Rong Li) ； 姜子濤(Zi-Tao Jiang) ； 劉雲浩(Yun-Hao Liu)

《藥物食品分析》 16卷5期 (2008/10) Pp. 91-96

摘要

本文報導了一個靈敏的固體相光度法測定清凉飮料中微量檸檬黃的新方法。在這個方法中，所利用的β-環糊精聚合物（60-80目）和離子交换樹脂相比，在紫外和可見光區吸收很小，其作爲固體相的支持體，並用於從樣品溶液中分離收集檸檬黃，然後在435nm波長下直接用於測定。檸檬黃的最佳分離條件是pH 1.0、20℃、反應時間40 min。所提出的方法用於清凉飮料中檸檬黃的測定，結果滿意。

關鍵字

檸檬黃 ； β-環糊精聚合物 ； 固體相分光光度法

並列摘要

A sensitive and new solid-phase spectrophotometry for the determination of tartrazine in soft drinks has been developed. In this method, β-cyclodextrin epichlorohydrin polymer (β-CDP) of 60-80 mesh, which has less background absorbance in ultraviolet and visible region compared to other ion-exchanger resins, was used to support direct adsorption of tartrazine from aqueous solutions to form a solid complex (tartrazine-β-CDP complex). The effects of pH, temperature, time and ionic strength on the β-CDP including tartrazine were determined. The optimum conditions were obtained (pH, 1.0; temperature, 20℃; time, 40 min). The method was applied for the direct solid phase spectrophotometric determination of tartrazine in soft drink samples at the maximum absorption wavelength of 435 nm with satisfactory results.

並列關鍵字

Tartrazine ； solid-phase spectrophotometry ； β-cyclodextrin epichlorohydrin polymer β-CDP