Inhibitory Effects of Polygonum cuspidatum on the Epstein-Barr Virus Lytic Cycle

虎杖抑制EB病毒溶裂循環

游進益(Ching-Yi Yiu) ； 陳師瑩(Shih-Ying Chen) ； 黃靖雯(Ching-Wen Huang) ； 葉東柏(Dong-Bor Yeh) ； 林翠品(Tsuey-Pin Lin)

《藥物食品分析》 19卷2期 (2011/06) Pp. 107-113

摘要

虎杖是在亞洲被廣泛使用的中草藥。本研究主要探討虎杖根乙醇萃取物是否能抑制EB病毒溶裂循環。細胞存活率以MTT[3-(4, 5-dimelhyl-2-thiazolyl)-2, 5-diphcnyl-2H-tetrazolium bromide]方法测定；EB病毒溶裂循環期蛋白質的表現真以流式細胞儀分析；以Real-time PCR方法分析病毒颗粒的释放：轉染含有EB病毒溶裂循環期的極早期基因，BRLF1及BRLF1啟動子及報導基因質體至P3HR1循環細胞分析病毒容裂循環捶早期基囚的轉錄。結果顯示虎杖根乙醇萃取物能抑制EB病毒柽早期基因的轉錄，也可以抑制EB病毒溶裂期蛋白質的表現，包括Rta、Zta及EA-D蛋白質的表現：進而也能降低EB病毒顆粒的產生。换言之，虎杖根乙醇革取物有應用在避免BE病毒擴大增殖的價值。

關鍵字

虎杖 ； 抗病毒 ； EB病毒

並列摘要

Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethanolic extract of Polygonum cuspidatum roots (PeE) for their capacity to inhibit the EBV lytic cycle. The cell viability was determined by the MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoliuni bromide] method. The expression of Epstein-Barr virus (EBV) lytic proteins was analyzed by flow cytometric assay. Real-time quantitative FUR was used to measure the EBV virion production. Transient transfection analysis was subsequently performed to assess the transcription of lytic genes, including BRLFI and BZLF1. Results showed that the PeE inhibited the transcription of EBV immediate early genes, the expression of EI3V lytic protetns, including Rta, Zta, and EA-D and reduced the production of virus particles, showing that PeE is useful for preventing the proliferation of the virus.

並列關鍵字

Polygonum cuspidatum ； antiviral activity ； EBV