Mutation of Histidine Synthesis Induced by Filamentous Phage Cf16 in Xanthomonas campestris pv. citri

柑桔潰瘍病菌由Cf16絲狀噬菌體誘導的組氨酸合成突變

吳文川(Wen-Chuan Wu) ； 楊鳳香(Feng-Hsiang Yang)

《植物病理學會刊》 3卷1期 (1994/03) Pp. 1-8

https://doi.org/10.6649/PPB.199403_3(1).0001

摘要

柑桔潰瘍病菌(Xanthomonas campestris pv. citri)的XW45菌株，被Cf16絲狀噬菌體潛溶化後，以約爲10^(-1)的頻率產生His^-突變菌株。全部或部分His^-突變菌株，XW45野生型菌株不同，不能利用檸檬酸鹽，不能分解海藻糖、木糖、甘露醇、廿露糖、甘油、果糖和乳糖，且其對柳橙的致病力稍微降低，而部份His^-突變菌株仍然不能在3%氯化鈉的培養基上生長。當此等His^-突變菌株回復時，未失去其潛溶性，而大部分His^-回復菌株依然保持此等變化的特性。XW45野生型菌株所含有pXW45N和pXW45J二個質體，在有些His^-突變菌株及His^+回復菌株細胞內，能融合成爲另一質體或再分裂成爲原來的質體。Cf16噬菌體在His^-突變菌株和His^+回復菌株細胞內均不呈顯游離質體狀態的DNA；因此，可推測具原噬菌體可能挿入寄主染色體受影響基因以外的一定位置上。

關鍵字

柑桔潰瘍病菌 ； Cf16絲狀噬菌體 ； 突變

並列摘要

Filamentous phage Cf16 induced mutation of histidine synthesis in strain XW45 of Xanthomonas campestris pv. citri at the frequency of 10 ^(-1) after lysogenization. All or most of the His^- mutants, unlike their wild-type strain XW45, did not utilize citrate, did not decompose the carbohydrates tested, and showed slight decrease in virulence to Licheng sweet orange; one of them was not tolerant to 3% NaCl. All the His^- mutants, when reverted, were still lysogenic for Cf16, and most of the His^+ revertants did not restore these alterated phenotypic expressions. The wild-type strain XW45 harbored plasmids pXW45N and pxw45J, which fused to become a large plasmid and then dissociated into two plasmids in some of the His mutants and the His^+ revertants. The Cf16 prophage was not observed existing as plasmid state in the cells of His mutants and His^+ revertants. Thus, the mutation of histidine synthesis in X. campestris pv. citri XW45 induced by Cf16 is probably due to integration of its prophage at a certain site other than the affected genes on the host chromosome.

並列關鍵字

Xanthomonas campestris pv. citri ； filamentous phage Cfi6 ； mutation.