Control of Turf Grass Seedborne Pathogenic Fungi by Ozone

本研究利用臭氧來防治草種種媒病原真菌Bipolaris australiensis, Curvularia pallescens 和Exserohilum rostratum，利用臭氧氣體在240 ppb濃度連續六天、每天四小時燻蒸處理無法減少種子帶菌率或增加種子發芽率。臭氧飽和水溶液則可有效抑制這三種真菌分生孢子之發芽，統計上臭氧水處理時間和分生孢子發芽之抑制率之相關性為多項式回歸關係。臭氧飽和水溶液導致B .australiensis, C. pallescens 和E. rostratum分生孢子100% 致死率處理時間分別為10, 13 和30分鐘，顯示三種供試真菌中B. australiensis 對臭氧最敏感，而E. rostratum 最具耐性，三種真菌中之細胞壁也以E. rostratum最厚。臭氧水並無法完全消除草種上之病菌，但可降低種子發芽時之病菌纏據率，並因而提高發芽率。盆栽試驗比較結果顯示臭氧水、生物防治製劑(Trichoderma sp. 和Bacillus megaterium) 與兩種傳統化學藥劑對三種種媒真菌具同等之防治效果，唯經臭氧水處理之種子，其幼苗生長勢顯較其他處理為佳，本文一併討論以臭氧水處理在種傳病害防治上之應用潛力。

關鍵字

臭氧水；草種種媒真菌；防治

並列摘要

Ozone was evaluated to reveal its efficacy for controlling turf grass seedborne pathogenic fungi, Bipolaris australiensis, Curvularia pallescens and Exserohilum rostratum. Fumigation of seeds infested by these fungi with ozone at 240 ppb for 6 consecutive days (4 hr/day) failed to increase seed germination. Ozone-saturated water, however, was found to be highly inhibitory on the conidial germination of three tested fungi. The correlation curve between percent inhibition of conidial germination and duration of treatment in ozonated water was polynomial regression for all three fungi. The duration needed to completely kill the conidia of B. australiensis, C. pallescens and E. rostratum was approximately 10, 13 and 30 min, respectively. E. rostratum was obviously the most tolerant to ozone; because its conidia have the thickest wall as comparing to that of other tested fungi. The control efficacy of turf grass seedborne fungi by application of ozonated water was found as good as that by application of antagonistic Trichoderma sp. and Bacillus megasperium and by two conventional fungicides tested. The seedling growth vigor, however, was apparently better from seeds treated with ozonated water. The potential application of ozonated water in seed treatment is herein discussed.

並列關鍵字

ozonated water ； turf grass seedborne fungi ； control

國際替代計量

Control of Turf Grass Seedborne Pathogenic Fungi by Ozone

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