產氛綠膿桿茵對植物病原細菌生長之影響

Effect of Cyanogenic Pseudomonas aeruginosa on the Growth of Plant Pathogenic Bacteria

林宜賢(Y. H. Lin) ； 張世杰(S. C. Chang) ； 蔡均龍(Y. L. Tsai) ； 徐世典(S. T. Hsu) ； 黃秀珍(H. C. Huang) ； 曾國欽(K. C. Tzeng)

《植物病理學會刊》 13卷3期 (2004/09) Pp. 211-218

https://doi.org/10.6649/PPB.200409_13(3).0006

摘要

林宜賢、張世杰、蔡洵龍、徐世典、黃秀珍、曾國欽 2004. 產氦綠膿桿菌對植物病原細菌生長之影響. 植病會刊13:211-218.
由葉表分離之綠膿桿菌（P sendomonas aeruginosa常具有產生氨酸的特性，而螢光假單胞菌氨酸的產生為防治植物真菌性病害的機制之一。本研究利用密閉培養皿系統測試葉表分離之綠膿桿菌菌株對不同植物病原細菌在培養基上之生長，發現產氨能力強之菌株，除對Erwinia。hrysaothemi CAC7外，可完全抑制Agrobacterium tumefaciens RS3、erwinia carotovora subsp. carotovora、ZL1、Pseudomonas syringae PA5、Ralstonia solanacearum PS152及Xanthomonas axonopodis pv. vesicatoria XVT40之生長，且綠膿桿菌之產氨能力與抑菌程度具正相關。利用不產氨之P. putida YLFP44菌株，經轉形所得之產氤衍生菌株，來探討氨酸在抑菌作用之角色，結果顯示產氦衍生菌株Y44HI（帶有由P. aeruginosa WFPllr選殖之hcnABC基因，除對Erwinia chrysanthemi CAS7外，亦可完全抑制其他供試之植物病原細菌的生長，及完全抑制不同寄主來源的青枯病菌，但僅帶有選殖載體的衍生菌株Y44CI，則不能產生氦酸亦無抑菌之能力，表示產氤菌株之抑菌作用與其氤酸的產生有關。Y44HI衍生菌株對青枯病菌不僅抑制其生長，處理時間長時（5天後也有致死作用，此外並可造成菌落及細胞形態之改變。

關鍵字

綠膿桿菌 ； 氰酸 ； 植物病原細菌 ； 青枯病菌 ； 抑制生長

並列摘要

Lin, Y. H., Chang, S. C., Tsai, Y. L., Hsu, S. T., Huang, H. C., and Tzeng, K. C. 2004. Effect of cyanogenic Pseudomonas aeruginosa on the growth of plant pathogenic bacteria. Plant Pathol. Bull. 13:211 218. (Department of Plant Pathology, National Chung-Hsing University, Taichung, Taiwan;Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, Taiwan; Corresponding author, E-mail: [e-mail]; Fax: [phone])
Many strains of Pseudomonas aeruginosa from plants are cyanogenic, and the production of hydrogen cyanide (HCN) is one of the factors involved in the control of certain fungal diseases. The effect of cyanogenic Pseudomonas aeruginosa strains on the growth of plant pathogenic bacteria was studied in a closed paired-plate system. Strong cyanogenic strains completely inhibited the growth of Agrobacterium tumefaciens RS3, Erwinia carotovora subsp. carotovora ZL1, Pseudomonas syringae PA5, Raistonia solanacearum PS 152 and Xanthomonas axonopodis pv. vesicatoria XVT4O, but not Erwinia chrysanthemi CAS7. The inhibitory activity was correlated with the HCN production ability of P. aeruginosa strains. The role of HCN in this growth inhibition was examined by testing the inhibitory ability of a cyanogenic transformant derived from noncyanogenic strain YLFP44 of P. putida. The results showed that P. putida derivative Y44H1 (harboring the cloned hcnABC genes from P. aeruginosa WFP1 ir) capable of producing HCN was able to inhibit the growth of different strains of R. solanacearum, whereas derivative Y44C1 (noncyanogenic, harboring only the cloning vector) was not, indicating the growth inhibition by the cyanogenic strain was related to the production of HCN. Prolonged treatment (5 days) with Y44H1 caused death of R. solanacearum and also altered the colony type and cell morphology of R. solanacearum.

並列關鍵字

Pseudomonas aeruginosa ； hydrogen cyanide ； plant pathogenic bacteria ； Ralstonia solanacearum ； growth inhibition