摘要
本研究探討花粉加馬線滅菌所需之輻射劑量及花粉中主要微生物菌株之輻射抗性。所取之花粉樣品為新竹區蜂農採集、烘乾處理過之雜花粉，鈷六十加馬線照射於清華大學原科中心同位素組鈷六十熱室中進行。結果顯示花粉樣品中微生物含量為10^3~ 10^4 CFU/g，主要為好氣性細菌、真菌及酵母菌。花粉中主要分離真菌菌株經鑑定為Pithomyces sp.，細菌菌株鑑定為Bacillus cereus，加馬輻射對培養於Sacc's培養基上之Pithomyces sp.菌株分生孢子之百分之九十致死劑量( L D_(90))為4.2 kGy，對培養於tryptic soy broth培養基之B. cereus的營養態及孢子態菌體之L D_(90)分別為0.9 kGy及6.2 kGy。上述所測花粉中之主要菌株皆非特殊高抗輻射菌株，以10 kGy之加馬輻射劑量照射對所採花粉樣品可達完全滅菌效果。
並列摘要
This study examined the lowest dose of irradiation needed for sterilization of honeybee-collected pollen and the radiation resistance of the microbial strains isolated from pollen. Pollen samples were harvested and dried by artificial heating by beekeepers in Hsinchu. Samples were irradiated in a cobalt-60 hot cell of the Isotope Division, Nuclear Science Technology Development Center, National Tsing Hua U n i v e r s i t y. Experimental results indicated that microbial populations in the commercial pollen ranged from 10^3 to 10^4 CFU/g. Among the microbiological parameters, a high aerobic counts, fungi, and yeasts were found. The predominant strains isolated from pollen were P i t h o m y c e s sp. and Bacillus cere u s. The 90% lethal dose (LD_(90))of gamma radiation in conidia of Pithomyces sp. was 4.2kGy. The LD_(90) of the vegetative cells and endospores of B. cereus were 0.9 kGy and 6.2 kGy respectively. The microbial strains isolated from pollen were not the high radiation resistant ones. 10kGy of gamma irradiation was necessary for sterilizing the microbes in the commercial production pollen.
延伸閱讀
- L., M. D., Chang, H. H., L., C. R., L., H. T., Scott, E. R., & Xiaojuan, H. (2009). 超寄生性真菌Coniothyrium minitans之葉面噴灑策略及與花粉混合使用對加拿大西部菜豆上菌核病（Sclerotinia sclerotiorum）之防治效果評估. 植物保護學會會刊, 51(1&2), 1-17. https://doi.org/10.6715/PPB.200906_51(1_2).0001
- 袁華靖（2016）。篩選具抑制花生根瘤線蟲(Meloidogyne arenaria)的內生細菌以開發成新穎之生物農藥〔碩士論文，國立虎尾科技大學〕。華藝線上圖書館。https://www.airitilibrary.com/Article/Detail?DocID=U0028-2305201622272900
- Kedves, M., Pardutz, A., & Varga, A. (1996). 花粉與微生物交互作用之超顯微構造的研究. TAIWANIA, 41(1), 43-52. https://doi.org/10.6165/tai.1996.41.43
- 楊啟春、陳朝棟（1974）。天然物之研究 第六報 麴黴代謝過程中有機化合物之研究。化學，(63年4)，107-110。https://doi.org/10.6623/chem.1974019
- 劉如芸（2006）。六種化學品系土肉桂葉子精油抗細菌、腐朽菌、病媒蚊幼蟲及室塵蟎活性〔碩士論文，國立臺灣大學〕。華藝線上圖書館。https://doi.org/10.6342/NTU.2006.00304