進行溫室與田間試驗,以測定32種拮抗真菌與細菌對防治扁豆 (Lens culinaris) 莖與花灰黴病 (Botrytis cinerea) 的防治效果。結果顯示,所有菌株在開花初期噴布扁豆全株均有降低灰黴病罹病率與罹病度的效果。其中4個菌株,包括 Pantoea agglomerans, Pseudomonas Fluorescens, Penicillium griseofulvum 及 Trichoderma hamatum,在溫室實驗時,可以增加扁豆的產量與降低灰黴病感染率。在 1998-2000 年田間試驗時,與對照接種處理比較,此四個菌株可以顯著的降低莖與花黴病的罹病度,而且其處理的產量顯著增加。其中以 Trichoderma hamatum 的效果最佳且最穩定。本試驗認為“葉面噴布拮抗微生物”為防治扁豆莖與花灰黴病的一種有效方法。
Greenhouse and field studies were conducted to assess the effectiveness of 32 antagonistic isolates of bacteria and fungi for control of stem and blossom blight of lentil caused by Botrytis cinerea. All of the isolates were effective in reducing the incidence and severity of the disease in the greenhouse when applied as foliar sprays at the early bloom stage. Four of the isolates including Pantoea agglomerans, Pseudomonas fluorescens, Penicillium griseofulvum and Trichoderma hamatum tested in the greenhouse, increased the seed yield and reduced the rate of seed infection by B. cinerea. In field experiments of 1998-2000, the same four isolates significantly reduced incidence and severity of botrytis stem and blossom blight of lentil, and increased seed yield compared to the Botrytis-inoculated control. Trichoderma hamatum was the most consistent among these four agents. This study suggests that foliar application of microbial antagonists may be an effective measure for the control of botrytis stem and blossom blight of lentil.