- 期刊
Antimicrobial Activity of Medicinal Plants Used by Indigenous People in Taiwan
台灣原住民常用之藥用植物抽出物的抗菌活性
摘要
Extracts derived from fresh and dry tissues of 14 plant species used in ethnobotanical medicine by the indigenous Tsou people in Taiwan were evaluated for activities against 10 species of plant pathogens, including seven fungi: Alternaria brassicicola, Colletotrichum higginsianum, Cylindrocladium scoparium, Fusarium moniliforme, Phytophthora infestans, Pythium aphanidermatum, and Rhizoctonia solani and three bacteria: Erwinia carotovora subsp. carotovora, Pseudomonas syringae, and Xanthomonas campestris pv. vesicatoria. Results showed that inhibition of spore germination, mycelial growth, and cell proliferation of the plant pathogens varied with plant extracts, microorganisms and extraction methods. Hot water extracts of fresh tissues of Solanum nigrum (SN) at 20-fold dilutions completely inhibited spore germination of A. brassicicola. Ethanol extracts from fresh and dry tissues of SN inhibited spore germination of A. brassicicola at the concentrations of 160-fold and 400-fold dilutions, respectively. In inoculation study, the severity of black leaf spot of crucifer caused by A. brassicicola was significantly reduced to below 27% for the treatments of extracts from 10% (w/v) of fresh and 1% (w/v) of dry tissues of SN, compared to the severity of 97.2% in water and ethanol controls. The water extract from 10% (w/v) of fresh Pueraria montana tissues was the most effective treatment against anthracnose of crucifer caused by C. higginsianum and the disease severity was 22.2%, compared to 94.4% for the untreated controls. Although both ethanol and water extracts from fresh tissues of Clematis tashiroi completely suppressed the lesion development of tomato late blight caused by P. infestans at a concentration of 10% (w/v), same suppressive effects at lower concentrations were observed only in the treatments of 2.5% (w/v) water extract from fresh tissues and 0.5% (w/v) water extract from dry tissues. These results suggest the possibility of control of plant diseases by natural substances from some ethnobotanical medicinal plants used by the indigenous people in Taiwan.
並列摘要
Extracts derived from fresh and dry tissues of 14 plant species used in ethnobotanical medicine by the indigenous Tsou people in Taiwan were evaluated for activities against 10 species of plant pathogens, including seven fungi: Alternaria brassicicola, Colletotrichum higginsianum, Cylindrocladium scoparium, Fusarium moniliforme, Phytophthora infestans, Pythium aphanidermatum, and Rhizoctonia solani and three bacteria: Erwinia carotovora subsp. carotovora, Pseudomonas syringae, and Xanthomonas campestris pv. vesicatoria. Results showed that inhibition of spore germination, mycelial growth, and cell proliferation of the plant pathogens varied with plant extracts, microorganisms and extraction methods. Hot water extracts of fresh tissues of Solanum nigrum (SN) at 20-fold dilutions completely inhibited spore germination of A. brassicicola. Ethanol extracts from fresh and dry tissues of SN inhibited spore germination of A. brassicicola at the concentrations of 160-fold and 400-fold dilutions, respectively. In inoculation study, the severity of black leaf spot of crucifer caused by A. brassicicola was significantly reduced to below 27% for the treatments of extracts from 10% (w/v) of fresh and 1% (w/v) of dry tissues of SN, compared to the severity of 97.2% in water and ethanol controls. The water extract from 10% (w/v) of fresh Pueraria montana tissues was the most effective treatment against anthracnose of crucifer caused by C. higginsianum and the disease severity was 22.2%, compared to 94.4% for the untreated controls. Although both ethanol and water extracts from fresh tissues of Clematis tashiroi completely suppressed the lesion development of tomato late blight caused by P. infestans at a concentration of 10% (w/v), same suppressive effects at lower concentrations were observed only in the treatments of 2.5% (w/v) water extract from fresh tissues and 0.5% (w/v) water extract from dry tissues. These results suggest the possibility of control of plant diseases by natural substances from some ethnobotanical medicinal plants used by the indigenous people in Taiwan.
延伸閱讀
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