光觸媒二氧化鈦薄膜對茭白細菌性基腐病原菌(Enterobacter cloacae SM1)之抑菌作用

Photocatalytic Inactivation of Enterobacter cloacae SM1 by TiO2 Thin Film under UV-A Light Irradiation in vitro

姚國山(K. S. Yao) ； 汪大永(D. Y. Wang) ； 顏仲志(J. J. Yan) ； 曾國欽(K. C. Tzeng)

《植物病理學會刊》 14卷4期 (2005/12) Pp. 265-268

https://doi.org/10.6649/PPB.200512_14(4).0006

摘要

為解決化學農藥所帶來的殘毒及環境污染問題，尋求新的替代作物病害防治方法，已成為防範植物病害重要的課題。近年來，利用紫外光A(UV-A)與二氧化鈦(TiO2)奈米粒子之光觸媒殺菌(photocatalytic inactivation)技術能有效的殺滅微生物且不會形成有害物質，應是一種可以嘗試的替代防治方法。Enterobacter cloacae SM1主要引起茭白細菌性基腐病，且造成茭白作物栽培之損失。本文利用溶膠凝膠法(sol-gel)製備二氧化鈦奈米薄膜，探討光觸媒二氧化鈦薄膜對茭白細菌性基腐病菌之抑菌效果，並以2,3,5-triphenyl-tetrazolium chloride(TTC)作為細菌生長指示劑。實驗結果顯示二氧化鈦薄膜經UV-A 30分鐘照射後，其對茭白細菌性基腐病菌與大腸桿菌(Escherichia coli BCRC 10450)之殺菌率分別達26.3%與31.8%，而經60分鐘照射後，對茭白細菌性基腐病菌與大腸桿菌之殺菌率皆達99.5%以上。因此，利用光觸媒二氧化鈦薄膜技術對植物病原菌之抑菌作用，將具有作為未來水耕栽培系統之植物病害防治新工具的潛力。

關鍵字

光觸媒殺菌 ； 二氧化鈦 ； 茭白細菌性基腐病菌

並列摘要

To solve the problems of synthetic pesticide residues and environmental pollutions, it is necessary to obtain an alternative method for the control of plant diseases. In recent years, the technology of photocatalytic inactivation of microorganisms with UV-A/TiO2, has been proposed as an innovative method, because it does not produce toxic compounds and has high efficiency of disinfection. It has been reported that Enterobacter cloacae SM1 causes bacterial basal rot of Zizania latifolia and leads to product losses. Photocatalytic inactivation of E. cloacae SM1 was therefore measured by TiO2 thin films prepared by sol-gel process on glass substrates. The bactericidal effect was examined using 2,3,5-triphenyl-tetrazolium chloride (TTC) as a bacterial growth indicator. The results showed that the inhibition rate of TiO2 thin film against bacteria of E. cloacae SM1 and Escherichia coli irradiation with UV-A (360nm) for 30min was 26.3% and 31.8%, respectively. However, after 60min irradiation the bactericidal rate of thin film was more than 99.5% for tested bacteria. Therefore, the photocatalytic inactivation of phytopathogen with TiO2 thin films has potential as a new tool for plant protection of hydroponics in the future.

並列關鍵字

Photocatalytic inactivation ； TiO2 ； Enterobacter cloacae SM1