在實驗室,將化學藥劑添加於培養基中,再測定菌絲生長與分生孢子發芽的情形,以評估41種藥劑於不同濃度下對檬果、文旦及香蕉炭疽病菌的抑制效果。在菌絲生長方面,化學藥劑添加於2% V-8蔬菜汁瓊脂(2% V-8A)供試時,分別有3種、18種及16種藥劑可以完全抑制檬果、文旦及香蕉炭疽病菌株之菌絲生長,其中以4-4式波爾多液、撲克拉乳劑及撲克拉錳可濕性粉劑等3種藥劑之抑制效果最佳,當有效成份濃度在10 mg/L時即可完全抑制所有供試菌株之菌絲生長。在孢子發芽方面,將化學藥劑添加於1% CV-8蔬菜汁培養液(1% CV-8B)中供試時,分別有8種、9種及8種藥劑可以完全抑制檬果、文旦及香蕉炭疽病菌株之分生孢子發芽,其中以4-4式波爾多液、鋅錳邁克尼可濕性粉劑、免得爛水分散陸粒劑、腈硫醌水懸劑、快得寧水懸劑、腐絕快得寧可濕性粉劑、嘉賜快得寧可濕性粉劑、鋅錳邁克尼可濕性粉劑等8種藥劑的藥效最佳,當有效成你濃度在10 mg/L時即叫完全抑制所有供試三種菌株之孢子發芽與形成附著器,此外賽普護汰寧雖然不能完全抑制孢子發芽,但孢子發芽後不易形成附著器。
Anthracnose caused by Colletotrichum gloeosporioides on mango and pomelo, and C. musae on banana were major fruit diseases in Taiwan. In the study, 2% V-8 juice agar (2% V-8A) or 1% CV-8 juice broth (1% CV-8B) were amended with individual chemical to evaluate the suppression ability of a total of 41 chemicals to anthracnose pathogens isolated from three different hosts. For suppression of mycelial growth on 2% V-8A, 3, 18 and 16 kinds of chemicals, respectively, showed strongly inhibition effects to mango, pomelo and banana isolates at all tested concentrations (10,100 and 1000 mg/L, ai). Especially, 3 chemicals were most effective; none of tested isolates could grow on the medium amended with them. They were 4-4 Bordeaux mixtures, 25% prochloraz EC and 50% prochlorate manganese WP. For inhibition of conidial germination in 1% CV-8B, 8, 9 and 8 kinds of chemicals, respectively, could completely suppress the conidia of all tested isolates from mango, pomelo and banana at all tested concentrations. Among them, 8 chemicals, including 4-4 Bordeaux mixtures, 80% mancozeb WP, 80% Metiram WG, 42.2% Dithianon SC, 33% oxine-copper SC, 53%Thiabenazole+Oxine-copper WP, 43.5% kasugamycin+oxine-copper WP, and 62.5% Myclobutanil + Mancozeb WP could completely inhibit conidial germination as well as appressoria formation of all tested isolates at all tested concentrations. 62.5%% Cyprodinil + Fludioxonil WG was also very effective in inhibiting appressoria formation but not conidial germination at low concentration.