Comparative genomics and phylogenetic analysis of Fusarium oxysporum species complex particularly on Fusarium oxysporum f. sp. cubense tropical race 4

香蕉黃葉病菌第四生理小種等尖孢鐮刀菌複合種的比較基因體學與類緣分析

周子禾(Tzu-Ho Chou) ； 曾顯雄(Shean-Shong Tzean)

《植物病理學會刊》 24卷1期 (2015/03) Pp. 25-35

https://doi.org/10.6649/PPB.201503_24(1).0003

摘要

Fusarium oxysporum f. sp. cubense (E. F. Sm.) W. C. Snyder & H. N. Hansen是引起香蕉黃葉病（Panama disease）之病原菌，該病原菌可分成四個生理小種，對香蕉生產造成了很嚴重的危害。目前香蕉黃葉病菌第四生理小種是台灣主要的香蕉黃葉病病原。研究此病菌的方法多樣，惟利用已完成之基因體序列及生物資訊學方法仍付闕如。本研究利用比較基因體學及類緣分析，找出第四生理小種有別於第一生理小種的基因共27個，類緣分析指出第一生理小種及第四生理小種感染香蕉的能力為一種趨同演化，以及找出九種鐮胞菌共同的基因共228個，可能可以當為共同防治的化學藥劑目標，但仍有待藥劑測試。此結果可作為進一步研究及同類型真菌後基因體時代分析的模式之一。

關鍵字

比較基因體學 ； 類緣分析 ； 鐮胞菌屬 ； 香蕉 ； 鐮胞菌萎凋病

並列摘要

Fusarium wilts are important crop diseases. Fusarium oxysporum f. sp. cubense causing Panama disease of banana severely devastated the world banana industry. To explore the underlying mechanism of Fusarium oxysporum (FO) and host plant interaction and search to better target FO genes for control purpose, we performed comparative genomics and phylogenetics analysis of Fusarium wilt pathogens, especially emphasizing on F. o. f. sp. cubense race 4 (FOCR4). We Blast searching identified 27genes from F. o. f. sp. cubense race 4; one of them was unique in NCBI nonredundant (nr) database. Phylogenetic analyses using neighbor joining, maximum parsimony, maximum likelihood and Bayesian inference revealed the occurrence of recombination between formae speciales. FOCR1 was phylogenetically distant from either FOCR4 or F. o. f. sp. cubense tropical race 4 (FOCTR4), implicating a convergent evolution in terms of infection capacity to banana. We also identified 228 consensus genes in Fusarium verticillioides and other eight F. oxysporum species complex strains. Those genes or their products could be the potential targets used for better controlling Panama disease of banana. The vision obtained in this study can be used as a model for postgenomic era research to fungi alike.

並列關鍵字

comparative genomics ； phylogeny ； Fusarium ； banana ； Fusarium wilt