Influence of rotation timing for flapping wing flight on the flying lift has been experimentally investigated in this study. The results reveal that rotation timing has significant influence on the flying lift. The averaged flying lift increases for high rotation wing velocity. Based on the comparisons of flying lift, too late A-rotation (connecting from wing downward motion to upward one) is the most serious taboo for the motion design of the micro air vehicles with flapping wings. Too late B-rotation (connection from upward motion to downward one) should also be avoided.